Dark septate endophyte Anteaglonium sp. T010 promotes biomass accumulation in poplar by regulating sucrose metabolism and hormones.

IF 3.5 2区 农林科学 Q1 FORESTRY
Dehui Qu, Fanlin Wu, Yingtian Guo, Jin Zhang, Mengyuan Li, Lina Yang, Lei Wang, Hongyan Su
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Abstract

Plant biomass is a highly promising renewable feedstock for the production of biofuels, chemicals and materials. Enhancing the content of plant biomass through endophyte symbiosis can effectively reduce economic and technological barriers in industrial production. In this study, we found that symbiosis with the dark septate endophyte (DSE) Anteaglonium sp. T010 significantly promoted the growth of poplar trees and increased plant biomass, including cellulose, lignin and starch. To further investigate whether plant biomass was related to sucrose metabolism, we analyzed the levels of relevant sugars and enzyme activities. During the symbiosis of Anteaglonium sp. T010, sucrose, fructose and glucose levels in the stem of poplar decreased, while the content of intermediates such as glucose-6-phosphate (G6P), fructose-6-phosphate (F6P) and UDP-glucose (UDPG), and the activity of enzymes related to sucrose metabolism, including sucrose synthase (SUSY), cell wall invertase (CWINV), fructokinase (FRK) and hexokinase, increased. In addition, the contents of glucose, fructose, starch, and their intermediates G6P, F6P and UDPG, as well as the enzyme activities of SUSY, CWINV, neutral invertase and FRK in roots were increased, which ultimately led to the increase of root biomass. Besides that, during the symbiotic process of Anteaglonium sp. T010, there were significant changes in the expression levels of root-related hormones, which may promote changes in sucrose metabolism and consequently increase the plant biomass. Therefore, this study suggested that DSE fungi can increase the plant biomass synthesis capacity by regulating the carbohydrate allocation and sink strength in poplar.

暗隔内生菌 Anteaglonium sp. T010 通过调节蔗糖代谢和激素促进杨树的生物量积累。
植物生物质是一种极具潜力的可再生原料,可用于生产生物燃料、化学品和材料。通过内生菌共生提高植物生物质的含量,可以有效降低工业生产中的经济和技术壁垒。本研究发现,与暗色隔内生菌(DSE)Anteaglonium sp. T010共生能显著促进杨树的生长,增加植物的生物量,包括纤维素、木质素和淀粉。为了进一步研究植物生物量是否与蔗糖代谢有关,我们分析了相关糖类的水平和酶活性。在 Anteaglonium sp.T010共生期间,杨树茎干中的蔗糖、果糖和葡萄糖含量下降,而中间产物如葡萄糖-6-磷酸(G6P)、果糖-6-磷酸(F6P)和UDP-葡萄糖(UDPG)的含量以及与蔗糖代谢相关的酶,包括蔗糖合成酶(SUSY)、细胞壁转化酶(CWINV)、果糖激酶(FRK)和己糖激酶(HxK)的活性增加。此外,根中葡萄糖、果糖、淀粉及其中间产物 G6P、F6P 和 UDPG 的含量,以及 SUSY、CWINV、中性转化酶(NINV)和 FRK 的酶活性都有所增加,最终导致根生物量的增加。此外,在 Anteaglonium sp. T010 的共生过程中,根相关激素的表达水平也发生了显著变化,这可能会促进蔗糖代谢的变化,从而增加植物的生物量。因此,本研究表明,DSE 真菌可通过调节杨树的碳水化合物分配和吸收能力来提高植物生物量合成能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Tree physiology
Tree physiology 农林科学-林学
CiteScore
7.10
自引率
7.50%
发文量
133
审稿时长
1 months
期刊介绍: Tree Physiology promotes research in a framework of hierarchically organized systems, measuring insight by the ability to link adjacent layers: thus, investigated tree physiology phenomenon should seek mechanistic explanation in finer-scale phenomena as well as seek significance in larger scale phenomena (Passioura 1979). A phenomenon not linked downscale is merely descriptive; an observation not linked upscale, might be trivial. Physiologists often refer qualitatively to processes at finer or coarser scale than the scale of their observation, and studies formally directed at three, or even two adjacent scales are rare. To emphasize the importance of relating mechanisms to coarser scale function, Tree Physiology will highlight papers doing so particularly well as feature papers.
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