{"title":"The complete chloroplast genome sequence of <i>Isoetes baodongii</i> (Isoetaceae).","authors":"Lin Guo, Junwen Zhai, Yufeng Gu","doi":"10.1080/23802359.2024.2356128","DOIUrl":null,"url":null,"abstract":"<p><p><i>Isoetes baodongii</i> is a diploid species of Isoetaceae distributed in low altitude area, its megaspore ornamentation is similar to tetraploid species <i>I. sinensis</i>. We collected leaf material of <i>I. baodongii</i> and sequenced it for low depth whole genome sequence, then, a complete chloroplast genome of <i>I. baodongii</i> was assembled and annotated. This chloroplast genome has a circular structure of 145,494 bp in length with a GC content of 38.0%, comprising a large single copy (LSC) region of 91,860 bp, a pair of inverted repeat (IR) regions of 13,207 bp each, and a small single copy (SSC) region of 27,220 bp. 136 genes were annotated, including 84 protein-coding genes, 38 tRNA genes, and 8 rRNA genes. A maximum likelihood phylogeny tree was reconstructed after the sequences alignment, the result showed that <i>I. baodongii</i> formed a sister clade to the one clustered by <i>I. sinensis</i>, <i>I. taiwanensis</i> and <i>I. orientalis</i>. Although the chloroplast genome structure of <i>I. baodongii</i> is extremely similar to other species distributed in China, a well-supported phylogenetic relationship was reconstructed here, these results may provide new messages for further studies on phylogeny and evolution of vascular plant on the earth.</p>","PeriodicalId":18647,"journal":{"name":"Mitochondrial DNA. Part B, Resources","volume":null,"pages":null},"PeriodicalIF":0.5000,"publicationDate":"2024-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11107852/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mitochondrial DNA. Part B, Resources","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/23802359.2024.2356128","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0
Abstract
Isoetes baodongii is a diploid species of Isoetaceae distributed in low altitude area, its megaspore ornamentation is similar to tetraploid species I. sinensis. We collected leaf material of I. baodongii and sequenced it for low depth whole genome sequence, then, a complete chloroplast genome of I. baodongii was assembled and annotated. This chloroplast genome has a circular structure of 145,494 bp in length with a GC content of 38.0%, comprising a large single copy (LSC) region of 91,860 bp, a pair of inverted repeat (IR) regions of 13,207 bp each, and a small single copy (SSC) region of 27,220 bp. 136 genes were annotated, including 84 protein-coding genes, 38 tRNA genes, and 8 rRNA genes. A maximum likelihood phylogeny tree was reconstructed after the sequences alignment, the result showed that I. baodongii formed a sister clade to the one clustered by I. sinensis, I. taiwanensis and I. orientalis. Although the chloroplast genome structure of I. baodongii is extremely similar to other species distributed in China, a well-supported phylogenetic relationship was reconstructed here, these results may provide new messages for further studies on phylogeny and evolution of vascular plant on the earth.
期刊介绍:
This open access journal publishes high-quality and concise research articles reporting the sequence of full mitochondrial genomes, and short communications focusing on the physical, chemical, and biochemical aspects of mtDNA and proteins involved in mtDNA metabolism and interactions.