Prevalence and antibiotic resistance in bacterial isolates of dogs with ulcerative keratitis in São Paulo State, Brazil.

IF 1.7 4区农林科学 Q2 VETERINARY SCIENCES

Veterinary ophthalmology Pub Date : 2024-05-20 DOI:10.1111/vop.13224

Pamella A F Casemiro, Alexandre L Andrade, Marita V Cardozo, Romário A Rodrigues, Jaqueline A Silva, Márcia Marinho, Alessandra F C Nassar, Vanessa Castro, Guilherme H R Braz, Cinthya A Gujanwski, Ivan R M Padua, Paola C Moraes

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Abstract

Objective: Identify microorganisms present in canine eyes affected by ulcerative keratitis and assess its resistance profile to available antimicrobial drugs.

Methods: Samples were collected from 88 canine eyes that exhibited ulcerative keratitis. They were identified using MALDI-TOF and subjected to antimicrobial susceptibility testing by disk diffusion.

Results: Among the assessed subjects, brachycephalic dogs accounted for 74.48% (50/83) of the evaluated canines. Among the 88 evaluated eyes, 90.9% (80/88) showed positive cultures, with 11.33% (10/88) of the samples isolating more than one species of bacteria. Of all bacterial isolates identified (90), Gram-positive bacteria accounted for 63.33% (57/90), while Gram-negative bacteria constituted 36.66% (33/90), with predominance of Staphylococcus spp. at 35.55% (32/90) being, Staphylococcus pseudintermedius at 68.75% (22/32), and Pseudomonas aeruginosa at 15.55% (14/90), respectively. Staphylococcus spp. exhibited resistance to penicillin (89.29%), sulfadiazine and trimethoprim (60.71%), and tetracycline (67.86%), while doxycycline (88.89%), cefotaxime (85.71%), chloramphenicol (82.14%), gentamicin, and moxifloxacin (78.57%) showed the highest sensitivity rates. Pseudomonas aeruginosa displayed sensitivity (100%) to gentamicin and imipenem, and resistance (8.33%) to norfloxacin, ciprofloxacin, and cefepime. Similarly, the Enterobacteriaceae family showed higher sensitivity to amikacin and gentamicin (88.89%), imipenem (88.24%), and levofloxacin (87.5%), with pronounced resistance to amoxicillin-clavulanate (50%) and cefazolin (47.06%). This highlights multiresistance in 23.33% (21/90) of the isolates.

Conclusions: The most isolated species in canine ulcerative keratitis are S. pseudintermedius and P. aeruginosa. However, other species were also isolated, demonstrating diversity in ocular microbiota infection. There is a high-rate multidrug resistance associated with canine ulcerative keratitis. Nevertheless, these strains exhibited sensitivity to antimicrobials commonly used in veterinary ophthalmology.

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巴西圣保罗州溃疡性角膜炎犬细菌分离物中的流行率和抗生素耐药性。

目的：确定溃疡性角膜炎犬眼中存在的微生物，并评估其对现有抗菌药物的耐药性：确定受溃疡性角膜炎影响的犬眼中存在的微生物，并评估其对现有抗菌药物的耐药性：方法：从 88 只患有溃疡性角膜炎的犬眼中采集样本。方法：从 88 只患有溃疡性角膜炎的狗眼中采集样本，使用 MALDI-TOF 进行鉴定，并通过磁盘扩散法进行抗菌药物药敏试验：在评估对象中，肱犬占 74.48%（50/83）。在 88 只接受评估的眼睛中，90.9%（80/88）的培养结果呈阳性，其中 11.33%（10/88）的样本分离出一种以上的细菌。在所有鉴定出的细菌分离物（90 个）中，革兰氏阳性菌占 63.33%（57/90），革兰氏阴性菌占 36.66%（33/90），其中以葡萄球菌为主，占 35.55%（32/90），假中间葡萄球菌占 68.75%（22/32），铜绿假单胞菌占 15.55%（14/90）。葡萄球菌属对青霉素（89.29%）、磺胺嘧啶和三甲氧苄（60.71%）以及四环素（67.86%）具有耐药性，而对强力霉素（88.89%）、头孢他啶（85.71%）、氯霉素（82.14%）、庆大霉素和莫西沙星（78.57%）的敏感率最高。铜绿假单胞菌对庆大霉素和亚胺培南敏感（100%），对诺氟沙星、环丙沙星和头孢吡肟耐药（8.33%）。同样，肠杆菌科细菌对阿米卡星和庆大霉素（88.89%）、亚胺培南（88.24%）和左氧氟沙星（87.5%）的敏感性较高，对阿莫西林-克拉维酸（50%）和头孢唑啉（47.06%）的耐药性明显。23.33%（21/90）的分离株具有多重耐药性：结论：犬溃疡性角膜炎中分离出最多的菌种是伪中间葡萄球菌和铜绿假单胞菌。然而，也分离到了其他物种，这表明眼部微生物群感染具有多样性。犬溃疡性角膜炎具有较高的多药耐药性。尽管如此，这些菌株对兽医眼科常用的抗菌药仍表现出敏感性。

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来源期刊

Veterinary ophthalmology 农林科学-兽医学

CiteScore

2.70

自引率

37.50%

发文量

审稿时长

12-24 weeks

期刊介绍： Veterinary Ophthalmology is a peer-reviewed, international journal that welcomes submission of manuscripts directed towards academic researchers of veterinary ophthalmology, specialists and general practitioners with a strong ophthalmology interest. Articles include those relating to all aspects of: Clinical and investigational veterinary and comparative ophthalmology; Prospective and retrospective studies or reviews of naturally occurring ocular disease in veterinary species; Experimental models of both animal and human ocular disease in veterinary species; Anatomic studies of the animal eye; Physiological studies of the animal eye; Pharmacological studies of the animal eye.