Evaluation of the Effects of Whey Protein and Moringa Oleifera Leaves Extract Mixture on Osseointegration in Rabbits.

Q2 Biochemistry, Genetics and Molecular Biology

Iranian Biomedical Journal Pub Date : 2024-03-01 DOI:10.61186/ibj.4025

Nawar Bahjet Kamil, Nada M H Al-Ghaban

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Abstract

Background: Osteogenic, antioxidant and anti-inflammatory effects of Whey protein and M. oleifera gel prompted us to evaluate their role alone or in combination on osseointegration in rabbits.

Methods: In this study, 24 titanium implants were inserted in the femurs of six rabbits. One implant was placed without treatment, and another one was coated with a mixture of whey protein and M. oleifera gel for each side. The animals were divided into two groups of 2- and 6-week intervals and evaluated using histopathological and immunohistochemical techniques.

Results: Histological evaluation revealed a significant difference between the experimental and the control groups after two weeks in osteoblast and osteocyte counts. The experimental group had mature bone development after six weeks of implantation, while the control group had a woven bone. Immunohistochemical results showed that the experimental group, compared to the control group, exhibited early positive expression of osteoblast cells at two weeks after the experiment. Based on histopathological observations, the experimental group showed a tiny area of collagenous fiber in 6th week after the implantation.

Conclusion: A mixture of whey protein and M. oleifera could accelerate osseointegration and healing processes.

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评估乳清蛋白和油辣木叶提取物混合物对家兔骨结合的影响

背景：乳清蛋白和油橄榄叶凝胶的成骨、抗氧化和抗炎作用促使我们评估它们单独或联合使用对兔子骨整合的作用：在这项研究中，我们在 6 只兔子的股骨中植入了 24 个钛植入物。其中一个植入物未经处理，另一个植入物的两侧分别涂有乳清蛋白和油橄榄叶凝胶的混合物。动物被分为间隔 2 周和 6 周的两组，并使用组织病理学和免疫组化技术进行评估：组织学评估显示，两周后，实验组和对照组的成骨细胞和骨细胞数量存在显著差异。实验组在植入六周后骨发育成熟，而对照组的骨呈编织状。免疫组化结果显示，与对照组相比，实验组在实验两周后表现出成骨细胞早期阳性表达。根据组织病理学观察，实验组在植入后第 6 周出现了一小块胶原纤维区域：结论：乳清蛋白和油橄榄果实的混合物可加速骨结合和愈合过程。

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