Development and Validation of an LC-MS/MS Method for the Determination of Plasma and Red Blood Cell Omega Fatty Acids: A Useful Diagnostic Tool

Separations Pub Date : 2024-05-01 DOI:10.3390/separations11050140

L. Farczádi, M. Dobreanu, A. Huțanu, Silvia Imre

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Abstract

Background: LC-MS is an ever-increasingly used methodology for clinical applications. Due to the superior selectivity and sensitivity, in certain situations, it can offer an advantage or be the only option for diagnostics and biomonitoring applications. Methods: A high selectivity sensitive LC-MS/MS method was developed for direct quantification of free plasma polyunsaturated fatty acids as well as conjugated membrane polyunsaturated fatty acids, using isocratic reverse phase elution. A quick and simple sample purification method was used in order to ensure high-throughput analysis of biological samples. The method was validated with regard to selectivity, sensitivity, linearity, accuracy, precision, carryover, and recovery, as well as other relevant parameters. Results and Conclusions: The method was developed and validated with respect to all relevant parameters and was successfully used in a number of clinical diagnostics and biomonitoring applications. The simple sample purification process allowed for an easy learning curve for analysts and other users, while ensuring a low chance of systematic or random errors and thus reliable results usable in a clinical setting.

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开发并验证用于测定血浆和红细胞欧米茄脂肪酸的 LC-MS/MS 方法：一种有用的诊断工具

背景：在临床应用中，LC-MS 是一种越来越常用的方法。由于其卓越的选择性和灵敏度，在某些情况下，它可以为诊断和生物监测应用提供优势或成为唯一的选择。方法：采用等度反相洗脱法，开发了一种高选择性、高灵敏度的 LC-MS/MS 方法，用于直接定量检测血浆游离多不饱和脂肪酸以及共轭膜多不饱和脂肪酸。为了确保生物样品的高通量分析，该方法采用了一种快速简便的样品纯化方法。对该方法的选择性、灵敏度、线性、准确度、精密度、携带率、回收率以及其他相关参数进行了验证。结果与结论：该方法的开发和验证涉及所有相关参数，并成功应用于多项临床诊断和生物监测。样品纯化过程简单，便于分析人员和其他用户学习，同时确保系统误差或随机误差的发生率较低，从而确保在临床环境中得到可靠的结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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