Effect of 1, 25 dihydroxy v itamin D3 on T Helper 17 (TH17) cell pathways in diarrhea associated irritable bowel syndrome

IF 0.6 4区医学 Q4 PHARMACOLOGY & PHARMACY

Tropical Journal of Pharmaceutical Research Pub Date : 2024-05-10 DOI:10.4314/tjpr.v23i4.10

Yongjian Li, Yan Wang

{"title":"Effect of 1, 25 dihydroxy v itamin D3 on T Helper 17 (TH17) cell pathways in diarrhea associated irritable bowel syndrome","authors":"Yongjian Li, Yan Wang","doi":"10.4314/tjpr.v23i4.10","DOIUrl":null,"url":null,"abstract":"Purpose: To investigate the effect of 1, 25-dihydroxyvitamin D3 (1,25-(OH)2D3) on T-Helper 17 (TH17) cell pathways in diarrhea-associated irritable bowel syndrome (IBS-D). \nMethods: 120 children with IBS-D were treated with Smecta alone and Smecta in addition to 1,25-(OH)2D3 in Hangzhou Ninth Peoples Hospital, Hangzhou, China. Blood samples were taken to determine the levels of 1,25-(OH)2D3, IL-17, IFN-γ, IL-23, and IL-6, as well as Th17 cell ratio. Furthermore, IL-17 and RORγt expressions were evaluated. \nResults: Serum 1,25-(OH)2D3 levels decreased in IBS-D patients and were negatively correlated with IL-17 levels in serum and Th17 cells in peripheral blood. Treatment with 1,25-(OH)2D3 significantly reduced serum IFN-γ, IL-17, IL-23 and IL-6 levels in IBS-D patients (p < 0.05). Percentage of Th17 cells, and IL-17 and RORγt expression levels were significantly reduced in IBS-D patients after 1,25-(OH)2D3 treatment (p < 0.05). \nConclusion: This study shows that 1,25-(OH)2D3 reduces serum IFN-γ, IL-17, IL-23, IL-6 levels, Th17 cells and RORγt expression in IBS-D patients. Additional studies with larger sample sizes are required to validate these findings.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":null,"pages":null},"PeriodicalIF":0.6000,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical Journal of Pharmaceutical Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.4314/tjpr.v23i4.10","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}

引用次数: 0

Abstract

Purpose: To investigate the effect of 1, 25-dihydroxyvitamin D3 (1,25-(OH)2D3) on T-Helper 17 (TH17) cell pathways in diarrhea-associated irritable bowel syndrome (IBS-D). Methods: 120 children with IBS-D were treated with Smecta alone and Smecta in addition to 1,25-(OH)2D3 in Hangzhou Ninth Peoples Hospital, Hangzhou, China. Blood samples were taken to determine the levels of 1,25-(OH)2D3, IL-17, IFN-γ, IL-23, and IL-6, as well as Th17 cell ratio. Furthermore, IL-17 and RORγt expressions were evaluated. Results: Serum 1,25-(OH)2D3 levels decreased in IBS-D patients and were negatively correlated with IL-17 levels in serum and Th17 cells in peripheral blood. Treatment with 1,25-(OH)2D3 significantly reduced serum IFN-γ, IL-17, IL-23 and IL-6 levels in IBS-D patients (p < 0.05). Percentage of Th17 cells, and IL-17 and RORγt expression levels were significantly reduced in IBS-D patients after 1,25-(OH)2D3 treatment (p < 0.05). Conclusion: This study shows that 1,25-(OH)2D3 reduces serum IFN-γ, IL-17, IL-23, IL-6 levels, Th17 cells and RORγt expression in IBS-D patients. Additional studies with larger sample sizes are required to validate these findings.

查看原文本刊更多论文

1, 25 二羟维生素 D3 对腹泻相关肠易激综合征中 T Helper 17 (TH17) 细胞通路的影响

目的：研究1,25-二羟维生素D3（1,25-(OH)2D3）对腹泻相关性肠易激综合征（IBS-D）中T-Helper 17（TH17）细胞通路的影响。方法：在杭州市第九人民医院，120名IBS-D患儿分别接受了思密达单独治疗和思密达与1,25-(OH)2D3联合治疗。采集血样测定1,25-（OH）2D3、IL-17、IFN-γ、IL-23和IL-6的水平以及Th17细胞比例。此外，还评估了 IL-17 和 RORγt 的表达。研究结果IBS-D患者血清中1,25-（OH）2D3水平下降，并与血清中的IL-17水平和外周血中的Th17细胞呈负相关。使用 1,25-（OH）2D3 治疗可显著降低 IBS-D 患者的血清 IFN-γ、IL-17、IL-23 和 IL-6 水平（p < 0.05）。1,25-（OH）2D3治疗后，IBS-D患者的Th17细胞比例、IL-17和RORγt表达水平明显降低（P < 0.05）。结论本研究表明，1,25-（OH）2D3 可降低 IBS-D 患者的血清 IFN-γ、IL-17、IL-23、IL-6 水平、Th17 细胞和 RORγt 表达。要验证这些发现，还需要更多样本量更大的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Tropical Journal of Pharmaceutical Research 医学-药学

CiteScore

1.00

自引率

33.30%

发文量

490

审稿时长

4-8 weeks

期刊介绍： We seek to encourage pharmaceutical and allied research of tropical and international relevance and to foster multidisciplinary research and collaboration among scientists, the pharmaceutical industry and the healthcare professionals. We publish articles in pharmaceutical sciences and related disciplines (including biotechnology, cell and molecular biology, drug utilization including adverse drug events, medical and other life sciences, and related engineering fields). Although primarily devoted to original research papers, we welcome reviews on current topics of special interest and relevance.