Biophenolic compounds and metal ions associated with the antioxidant and antibacterial activity of the ethanolic extract of Heliotropium arborescens L. leaves from the Andean region of Ayacucho-Peru

Pharmacia Pub Date : 2024-05-14 DOI:10.3897/pharmacia.71.e120315

Angel T. Alvarado, H. Chávez, Jorge A. García, María R. Bendezú, Felipe Surco-Laos, Aura Molina-Cabrera, Doris Laos-Anchante, Nelly Vega-Ramos, Juan J. Palomino-Jhong, Paulina Eliades Yarasca-Carlos, B. Loja-Herrera, Mario Pineda-Pérez, Mario Bolarte-Arteaga

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Abstract

Heliotropium arborescens (Galán de Mera et al. 2022) is a herbaceous plant that contains various biophenolic compounds that exhibit antibacterial property and antioxidant activity that could prevent diseases caused by free radicals. The objective was to study the biophenolic compounds and metal ions associated with the in vitro antioxidant and antibacterial activity of the ethanolic extract of Heliotropium arborescens L. leaves from the Andean region of Ayacucho-Peru. The total polyphenolic content (TPC) was determined by the Folin-Ciocalteu method; Total flavonoid content (TFC) was determined by spectrophotometry at 510 nm, TFC was expressed in mg quercetin/g sample (mg QE/g). Antioxidant activity by three in vitro methods; determination of metals by flame atomic absorption spectrophotometry (FAAS) and antibacterial activity by agar diffusion method. The values obtained from the TPC were 306.80±0.05 mg GAE/g, TFC 111.53±0.25 mg QE/g. Antioxidant activity: DPPH IC50 37.55±0.04 µL and 65.64±0.10 µL, at 0.47 and 0.93 mg/mL extract, respectively; ABTS 0.20±0.01 mM TEAC/g (0.41 mg/mL extract) and 0.47±0.01 (0.82 mg/mL extract); FRAP 0.24±0.01 mM TEAC/g (0.94 mg/mL extract) and 0.46±0.02 mM TEAC/g (1.88 mg/mL extract). Correlation relationship TFC/DPPH (r=0.9994; R2 0.9989), TFC/ABTS (r = 0.9572; R2 0.9998) and TFC/FRAP (r = 09933; R2 0.9868). Calcium 1.22±0.04, copper 0.77±0.05, magnesium 851.7±3.06 and zinc 20.63±1.19 mg/100 g were found in all cases. Antibacterial activity on Bacillus subtilis (ATCC 6633) and Staphylococcus aureus (ATCC 25923) with a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) > 400 μg/mL. It is concluded that the total biophenolic components and metals of the ethanolic extract of the leaves of H. arborescens L. show potential antioxidant activity, and moderate antibacterial activity against Bacillus subtilis and Staphylococcus aureus. Graphical abstract:

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与阿亚库乔-秘鲁安第斯地区的 Heliotropium arborescens L. 叶子乙醇提取物的抗氧化和抗菌活性有关的生物酚类化合物和金属离子

Heliotropium arborescens（Galán de Mera 等人，2022 年）是一种草本植物，含有多种生物酚类化合物，具有抗菌特性和抗氧化活性，可预防自由基引起的疾病。本研究旨在研究阿亚库乔-秘鲁安第斯地区的 Heliotropium arborescens L. 叶子乙醇提取物中与体外抗氧化和抗菌活性相关的生物酚类化合物和金属离子。总多酚含量（TPC）用 Folin-Ciocalteu 法测定；总黄酮含量（TFC）用分光光度法在 510 纳米波长下测定，以毫克槲皮素/克样品（毫克 QE/克）表示。采用三种体外方法测定抗氧化活性；采用火焰原子吸收分光光度法（FAAS）测定金属含量；采用琼脂扩散法测定抗菌活性。从 TPC 中得到的值为 306.80±0.05 mg GAE/g，TFC 为 111.53±0.25 mg QE/g。抗氧化活性：DPPH IC50 分别为 37.55±0.04 µL 和 65.64±0.10 µL （提取物为 0.47 和 0.93 mg/mL）；ABTS 为 0.20±0.01 mM TEAC/g（提取物为 0.41 mg/mL）和 0.47±0.01（提取物为 0.82 mg/mL）；FRAP 为 0.24±0.01 mM TEAC/g（提取物为 0.94 mg/mL）和 0.46±0.02 mM TEAC/g（提取物为 1.88 mg/mL）。相关关系 TFC/DPPH (r=0.9994; R2 0.9989)、TFC/ABTS (r = 0.9572; R2 0.9998) 和 TFC/FRAP (r = 09933; R2 0.9868)。钙 1.22±0.04，铜 0.77±0.05，镁 851.7±3.06，锌 20.63±1.19 mg/100 g。对枯草芽孢杆菌（ATCC 6633）和金黄色葡萄球菌（ATCC 25923）具有抗菌活性，最低抑菌浓度（MIC）和最低杀菌浓度（MBC）大于 400 μg/mL。结论是树叶乙醇提取物的总生物酚类成分和金属具有潜在的抗氧化活性，对枯草杆菌和金黄色葡萄球菌具有中等程度的抗菌活性。图表摘要：

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