Optical lateral flow assays in early diagnosis of SARS-CoV-2 infection

IF 1.8 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences Pub Date : 2024-05-17 DOI:10.1007/s44211-024-00596-6

Rushi Liang, Aiping Fan, Feiqian Wang, Yajing Niu

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引用次数: 0

Abstract

So far, the 2019 novel coronavirus (COVID-19) is spreading widely worldwide. The early diagnosis of infection by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is essential to provide timely treatment and prevent its further spread. Lateral flow assays (LFAs) have the advantages of rapid detection, simple operation, low cost, ease of mass production, and no need for special devices and professional operators, which make them suitable for self-testing at home. This review focuses on the early diagnosis of SARS-CoV-2 infection based on optical LFAs including colorimetric, fluorescent (FL), chemiluminescent (CL), and surface-enhanced Raman scattering (SERS) LFAs for the detection of SARS-CoV-2 antigens and nucleic acids. The types of recognition components, detection modes used for antigen detection, labels employed in different optical LFAs, and strategies to improve the detection sensitivity of LFAs were reviewed. Meanwhile, LFAs coupled with different nucleic acid amplification techniques and CRISPR–Cas systems for the detection of SARS-CoV-2 nucleic acids were summarized. We hope this review provides research mentalities for developing highly sensitive LFAs that can be used in home self-testing for the early diagnosis of SARS-CoV-2 infection.

Graphical abstract

查看原文本刊更多论文

用于早期诊断 SARS-CoV-2 感染的光学横向流动测定。

到目前为止，2019 年新型冠状病毒（COVID-19）正在全球广泛传播。严重急性呼吸系统综合征冠状病毒-2（SARS-CoV-2）感染的早期诊断对于及时治疗和防止其进一步传播至关重要。侧流检测法（LFA）具有检测快速、操作简单、成本低廉、易于批量生产、无需特殊设备和专业操作人员等优点，适合在家中进行自我检测。本综述主要介绍基于光学 LFA 的 SARS-CoV-2 感染早期诊断方法，包括用于检测 SARS-CoV-2 抗原和核酸的比色法、荧光法（FL）、化学发光法（CL）和表面增强拉曼散射法（SERS）LFA。综述了识别元件的类型、用于抗原检测的检测模式、不同光学 LFA 中使用的标签以及提高 LFA 检测灵敏度的策略。同时，综述了 LFA 与不同的核酸扩增技术和 CRISPR-Cas 系统联用检测 SARS-CoV-2 核酸的情况。我们希望这篇综述能为开发高灵敏度的 LFAs 提供研究思路，使其能用于 SARS-CoV-2 感染的早期诊断的家庭自我检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytical Sciences 化学-分析化学

CiteScore

2.90

自引率

18.80%

发文量

232

审稿时长

1 months

期刊介绍： Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods. This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.