P. Thomas , S. Sahoo , S. Mahajan , D.L. Hiremath , A.H. Udayashankara , S. Srivastava
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引用次数: 0
Abstract
Introduction
Resistance to chemoradiation therapy results in poor prognosis and relapse in a sizeable percentage of cervical cancer patients. Toxicities and long-term side effects associated with radiation also warrant the need for molecules that could sensitise cells to radiation therapy. Naturally-derived compounds are a reservoir of non-toxic, potentially effective radiosensitizers of therapy-resistant tumours. This study evaluates the antitumorigenic properties of Aloe vera extracts.
Methods
Phenotypic assays and biochemical experiments like flow cytometry, real-time quantitative PCR, immunofluorescent imaging, western blotting, migration, and wound-healing assays were used to determine the effect of Aloe vera on cervical cancer cell lines.
Results
While Aloe vera treatment caused no significant changes to proliferation, survival, and cell cycling profiles, the expression of periostin, a molecule known for its association with stem-like cancer cells, decreased significantly. Phenotypically, Aloe vera treatment led to diminished clonogenicity and migratory abilities. Finally, combinatorial treatment of cells with Aloe vera and atorvastatin (a pan-Rho GTPase inhibitor) before radiation therapy resulted in a marked decrease in cell survival.
Conclusions
Our findings prove that Aloe vera treatment leads to reduced expression of periostin and adversely affects self-renewal and migration. Importantly, this work provides evidence that Aloe vera, in combination with atorvastatin, could serve as potential radiosensitizers of cancer cells.
期刊介绍:
The Journal of Herbal Medicine, the official journal of the National Institute of Medical Herbalists, is a peer reviewed journal which aims to serve its readers as an authoritative resource on the profession and practice of herbal medicine. The content areas of the journal reflect the interests of Medical Herbalists and other health professionals interested in the clinical and professional application of botanical medicines. The objective is to strengthen the research and educational base of herbal medicine with research papers in the form of case studies, original research articles and reviews, monographs, clinical trials and relevant in vitro studies. It also publishes policy statements, opinion pieces, book reviews, conference proceedings and profession related information such as pharmacovigilance reports providing an information source for not only the Herbal Practitioner but any Health professional with an interest in phytotherapy.