Investigation of subclinical ocular inflammation in the aqueous humor of patients with myopia following bilateral sequential collamer lens implantation

IF 1.6 4区医学 Q4 IMMUNOLOGY

Transplant immunology Pub Date : 2024-05-13 DOI:10.1016/j.trim.2024.102052

Xiao-Qin Wang , Shi-Jie Zheng , Yong-Guo Xiang , Fan-Fan Huang , Rong-Xi Huang , Wen-Juan Wan , Ke Hu

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Abstract

Background

The aqueous humor, a transparent fluid secreted by the ciliary body, supports the lens of the eyeball. In this study, we analyzed the cytokine and chemokine profiles within the aqueous humor of the contralateral eye post-implantation of an implantable collamer lens (ICL) to evaluate potential subclinical inflammation in the second eye subsequent to ICL implantation in the first eye.

Methods

Aqueous humor samples were procured from both eyes of 40 patients (totaling 80 eyes) prior to bilateral ICL insertion. Subsequently, a comprehensive statistical analysis was conducted using the Luminex assay to quantify 30 different cytokines in these samples.

Results

Compared to the first eye, the aqueous humor of the second eye demonstrated decreased concentrations of IFN-γ (P = 0.038), IL-13 (P = 0.027), IL-17/IL-17 A (P = 0.012), and IL-4 (P = 0.025). No significant differences were observed in other cytokine levels between the two groups. Patients were then categorized based on the postoperative rise in intraocular pressure (IOP) in the first eye. The group with elevated IOP displayed elevated levels of EGF in the aqueous humor of the first eye (P = 0.013) and higher levels of PDGF-AB/BB in the aqueous humor of the second eye (P = 0.032) compared to the group with normal IOP. Within the elevated IOP group, the levels of EGF (P = 0.013) and IL-17/IL-17 A (P = 0.016) in the aqueous humor were lower in the second eye than in the first eye. In the normal IOP group, cytokine levels did not differ notably between eyes.

Conclusion

Following sequential ICL implantation, it appears that a protective response may be activated to mitigate subclinical inflammation in the second eye induced by the initial implantation in the first eye. Additionally, the increase in IOP subsequent to surgery in the first eye may correlate with the presence of inflammatory mediators in the aqueous humor.

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双侧连续性准分子晶体植入术后近视患者眼房水亚临床炎症的调查。

背景：房水是睫状体分泌的一种透明液体，支撑着眼球的晶状体。在这项研究中，我们分析了植入可摘除晶体（ICL）后对侧眼房水中的细胞因子和趋化因子谱，以评估第一只眼植入 ICL 后第二只眼可能出现的亚临床炎症：方法：在双侧植入 ICL 之前，从 40 名患者（共 80 眼）的双眼采集了房水样本。随后，使用 Luminex 检测法对这些样本中的 30 种不同细胞因子进行了综合统计分析：结果：与第一只眼睛相比，第二只眼睛的房水中 IFN-γ (P = 0.038)、IL-13 (P = 0.027)、IL-17/IL-17 A (P = 0.012) 和 IL-4 (P = 0.025) 的浓度有所下降。两组患者的其他细胞因子水平无明显差异。然后，根据术后第一只眼睛的眼压（IOP）升高情况对患者进行分类。与眼压正常组相比，眼压升高组第一只眼睛的房水中 EGF 水平升高（P = 0.013），第二只眼睛的房水中 PDGF-AB/BB 水平升高（P = 0.032）。在眼压升高组中，第二只眼房水中的 EGF 水平（P = 0.013）和 IL-17/IL-17 A 水平（P = 0.016）低于第一只眼。在正常眼压组，两只眼睛的细胞因子水平没有明显差异：结论：连续植入 ICL 后，第二只眼的保护性反应似乎会被激活，以减轻第一只眼初次植入 ICL 所引起的亚临床炎症。此外，第一只眼睛手术后眼压的升高可能与房水中存在的炎症介质有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Transplant immunology 医学-免疫学

CiteScore

2.10

自引率

13.30%

发文量

198

审稿时长

48 days

期刊介绍： Transplant Immunology will publish up-to-date information on all aspects of the broad field it encompasses. The journal will be directed at (basic) scientists, tissue typers, transplant physicians and surgeons, and research and data on all immunological aspects of organ-, tissue- and (haematopoietic) stem cell transplantation are of potential interest to the readers of Transplant Immunology. Original papers, Review articles and Hypotheses will be considered for publication and submitted manuscripts will be rapidly peer-reviewed and published. They will be judged on the basis of scientific merit, originality, timeliness and quality.