Investigating the anti-inflammatory and anti-arthritis effects of fucoidan from a brown seaweed

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Preethy P. Raj , Rajesh Kanna Gopal , Elumalai Sanniyasi
{"title":"Investigating the anti-inflammatory and anti-arthritis effects of fucoidan from a brown seaweed","authors":"Preethy P. Raj ,&nbsp;Rajesh Kanna Gopal ,&nbsp;Elumalai Sanniyasi","doi":"10.1016/j.crbiot.2024.100220","DOIUrl":null,"url":null,"abstract":"<div><p>Severe inflammation in joints caused by the detrimental effects of the immune system is termed Rheumatoid arthritis. The unconstrained proliferation of immune cells and pro-inflammatory cytokines deteriorates Synovium which secretes synovial fluid to lubricate joints and cartilage. Non-steroidal anti-inflammatory drugs (NSAIDs) are the only therapeutics for treating rheumatoid arthritis, and long-term intake causes serious side effects on the organs. Fucoidan, a sulfated polysaccharide found on the cell walls of brown algae shows bioactive potential. In our study, fucoidan was extracted from <em>Padina pavonica</em> (PD), <em>Stoechospermum marginatum</em> (StM), <em>Spatolossum macrodontum</em> (SpM), <em>Dictyota bartayresiana</em> (DD), and <em>Turbinaria decurrens</em> (TD) and evaluated for anti-inflammatory and anti-arthritis activities. Fucoidan was extracted and evaluated for anti-inflammatory activity <em>in vitro</em> using RAW 264.7 macrophage cell lines, followed by <em>in vivo</em> anti-arthritis activity on Wistar male rats. Nitric oxide suppression was comparatively high in fucoidan from TD (IC<sub>50</sub> − 12.93 µg/mL). Purified fucoidan from TD, significantly reduced inflammation, size of paw edema, downregulated proinflammatory cytokines (IL-6, IL-1β, TNF-α), and upregulated anti-inflammatory cytokine (IL10) in CFA-induced arthritis in Wistar male rats. Biochemical parameters like SOD, CAT, GSH, GPX, and GST and haematological parameters like total-protein, albumin, haemoglobin, and RBC were upregulated, and other parameters like urea, uric acid, creatinine, bilirubin, SGOT, SGPT, ALP, WBC, ESR, RF, and CRP were downregulated. Histopathology of the liver, kidney, and ankle joints reveals that fucoidan intake restrained inflammation and tissue damage. Therefore, fucoidan extracted from TD is a potential candidate for the treatment of rheumatoid arthritis.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":null,"pages":null},"PeriodicalIF":3.6000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000467/pdfft?md5=e3746c4e2d04e126ed16f7ed27d36772&pid=1-s2.0-S2590262824000467-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Research in Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2590262824000467","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Severe inflammation in joints caused by the detrimental effects of the immune system is termed Rheumatoid arthritis. The unconstrained proliferation of immune cells and pro-inflammatory cytokines deteriorates Synovium which secretes synovial fluid to lubricate joints and cartilage. Non-steroidal anti-inflammatory drugs (NSAIDs) are the only therapeutics for treating rheumatoid arthritis, and long-term intake causes serious side effects on the organs. Fucoidan, a sulfated polysaccharide found on the cell walls of brown algae shows bioactive potential. In our study, fucoidan was extracted from Padina pavonica (PD), Stoechospermum marginatum (StM), Spatolossum macrodontum (SpM), Dictyota bartayresiana (DD), and Turbinaria decurrens (TD) and evaluated for anti-inflammatory and anti-arthritis activities. Fucoidan was extracted and evaluated for anti-inflammatory activity in vitro using RAW 264.7 macrophage cell lines, followed by in vivo anti-arthritis activity on Wistar male rats. Nitric oxide suppression was comparatively high in fucoidan from TD (IC50 − 12.93 µg/mL). Purified fucoidan from TD, significantly reduced inflammation, size of paw edema, downregulated proinflammatory cytokines (IL-6, IL-1β, TNF-α), and upregulated anti-inflammatory cytokine (IL10) in CFA-induced arthritis in Wistar male rats. Biochemical parameters like SOD, CAT, GSH, GPX, and GST and haematological parameters like total-protein, albumin, haemoglobin, and RBC were upregulated, and other parameters like urea, uric acid, creatinine, bilirubin, SGOT, SGPT, ALP, WBC, ESR, RF, and CRP were downregulated. Histopathology of the liver, kidney, and ankle joints reveals that fucoidan intake restrained inflammation and tissue damage. Therefore, fucoidan extracted from TD is a potential candidate for the treatment of rheumatoid arthritis.

Abstract Image

研究褐藻中褐藻糖胶的抗炎和抗关节炎作用
免疫系统的有害影响导致的严重关节炎症被称为类风湿性关节炎。免疫细胞和促炎细胞因子的无限制增殖会使滑膜恶化,而滑膜分泌的滑液可以润滑关节和软骨。非甾体抗炎药(NSAIDs)是治疗类风湿性关节炎的唯一疗法,长期服用会对器官产生严重的副作用。褐藻糖胶是褐藻细胞壁上的一种硫酸化多糖,具有生物活性潜力。在我们的研究中,褐藻糖胶从 Padina pavonica (PD)、Stoechospermum marginatum (StM)、Spatolossum macrodontum (SpM)、Dictyota bartayresiana (DD) 和 Turbinaria decurrens (TD) 中提取,并对其抗炎和抗关节炎活性进行了评估。利用 RAW 264.7 巨噬细胞系提取并评估了褐藻糖胶的体外抗炎活性,然后评估了 Wistar 雄性大鼠体内的抗关节炎活性。褐藻糖胶对一氧化氮的抑制作用相对较高(IC50 - 12.93 µg/mL)。在CFA诱导的Wistar雄性大鼠关节炎中,从TD中纯化的褐藻糖胶能显著减少炎症、爪水肿的大小,下调促炎细胞因子(IL-6、IL-1β、TNF-α),上调抗炎细胞因子(IL10)。SOD、CAT、GSH、GPX 和 GST 等生化参数以及总蛋白、白蛋白、血红蛋白和红细胞等血液学参数被上调,尿素、尿酸、肌酐、胆红素、SGOT、SGPT、ALP、WBC、ESR、RF 和 CRP 等其他参数被下调。肝脏、肾脏和踝关节的组织病理学显示,褐藻糖胶的摄入抑制了炎症和组织损伤。因此,从TD中提取的褐藻糖胶是治疗类风湿性关节炎的潜在候选物质。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Current Research in Biotechnology
Current Research in Biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
6.70
自引率
3.60%
发文量
50
审稿时长
38 days
期刊介绍: Current Research in Biotechnology (CRBIOT) is a new primary research, gold open access journal from Elsevier. CRBIOT publishes original papers, reviews, and short communications (including viewpoints and perspectives) resulting from research in biotechnology and biotech-associated disciplines. Current Research in Biotechnology is a peer-reviewed gold open access (OA) journal and upon acceptance all articles are permanently and freely available. It is a companion to the highly regarded review journal Current Opinion in Biotechnology (2018 CiteScore 8.450) and is part of the Current Opinion and Research (CO+RE) suite of journals. All CO+RE journals leverage the Current Opinion legacy-of editorial excellence, high-impact, and global reach-to ensure they are a widely read resource that is integral to scientists' workflow.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信