Osteogenic potential of Frondoside A in human periodontal ligament cells: an RNA-Seq analysis.

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Periodontal and Implant Science Pub Date : 2024-04-17 DOI:10.5051/jpis.2303840192

Young Kim, Si-Won Kang, Ju Ri Ye, Seong Eun Kim, Yong Kwon Chae, Ok Hyung Nam

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引用次数: 0

Abstract

Purpose: The aim of this study was to evaluate the effects of Frondoside A (FA) on the osteogenic differentiation of human periodontal ligament (PDL) cells.

Methods: Human PDL cells were cultured in osteogenic medium and treated with FA at concentrations of 0, 0.05, and 0.2 µM for 14 days. The expression levels of genes associated with osteogenic differentiation were assessed using quantitative real-time polymerase chain reaction analysis. Subsequently, RNA sequencing was performed to identify enriched gene sets following FA treatment. Alkaline phosphatase (ALP) activity was measured to confirm the osteogenic potential of FA.

Results: Treatment with 0.2 µM FA significantly increased the expression levels of runt-related transcription factor 2 (RUNX2), ALP, and osteocalcin (OCN) at day 3, while also significantly elevating the expression of dentin sialophosphoprotein (DSPP), RUNX2, ALP, OCN, and osterix (OSX) at day 14 (P<0.017). Hallmark gene sets enriched during FA treatment were associated with the KRAS (normalized enrichment score [NES]=2.02, Q=0.000), interferon alpha (IFN-α) (NES=1.88, Q=0.001), IFN-γ (NES=1.85, Q<0.001), hypoxia (NES=1.79, Q=0.001), and p53 (NES=1.77, Q=0.001) signaling pathways. Additionally, treatment with 0.2 µM FA significantly intensified ALP staining at day 14 (P<0.05).

Conclusions: Within the limitations of this study, FA treatment influenced periodontal regeneration by promoting the osteogenic differentiation of human PDL cells.

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Frondoside A 在人类牙周韧带细胞中的成骨潜力：RNA-Seq 分析。

目的：本研究旨在评估佛手苷 A（FA）对人牙周韧带（PDL）细胞成骨分化的影响。方法：在成骨培养基中培养人 PDL 细胞，并用浓度为 0、0.05 和 0.2 µM 的 FA 处理 14 天。使用定量实时聚合酶链反应分析评估与成骨分化相关的基因表达水平。随后进行了 RNA 测序，以确定 FA 处理后富集的基因集。碱性磷酸酶（ALP）活性的测定证实了 FA 的成骨潜力：结果：用 0.2 µM FA 处理第 3 天时，可显著提高与 runt 相关的转录因子 2 (RUNX2)、ALP 和骨钙素 (OCN) 的表达水平；第 14 天时，可显著提高牙本质钙磷蛋白 (DSPP)、RUNX2、ALP、OCN 和 osterix (OSX) 的表达水平（PQ=0.000）、α干扰素（IFN-α）（NES=1.88，Q=0.001）、IFN-γ（NES=1.85，QQ=0.001）和 p53（NES=1.77，Q=0.001）信号通路。此外，用 0.2 µM FA 处理第 14 天时，ALP 染色显著增强（PConclusions：在本研究的局限性范围内，FA 处理通过促进人类 PDL 细胞的成骨分化影响了牙周再生。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Periodontal and Implant Science DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.30

自引率

5.30%

发文量

期刊介绍： Journal of Periodontal & Implant Science (JPIS) is a peer-reviewed and open-access journal providing up-to-date information relevant to professionalism of periodontology and dental implantology. JPIS is dedicated to global and extensive publication which includes evidence-based original articles, and fundamental reviews in order to cover a variety of interests in the field of periodontal as well as implant science.