A multiplex PCR assay to detect mislabelling in fish products.

IF 2.5 3区农林科学 Q2 CHEMISTRY, APPLIED

Food additives & contaminants. Part B, Surveillance Pub Date : 2024-09-01 Epub Date: 2024-05-10 DOI:10.1080/19393210.2024.2349606

Komal, Shahid Sherzada, Muhammad Imran, Saeed Akram Khan, Abdul Wajid

引用次数: 0

Abstract

Fish substitution in fish products is an important issue in fish markets, as it is a widespread practice. An authentication protocol for Rohu, Thaila and Tilapia was developed by multiplex PCR. Three species-specific and one degenerate common forward primer were designed using the Cytb gene of the mitochondrial genome. These primers for Labeo rohita, Labeo catla and Oreochromis niloticus showed the fragment size of 235 bp, 186 bp and 506 bp on the agarose gel, respectively. The primers for L. rohita and L. catla were sensitive to 0.1 ng of DNA template, while for O. niloticus this value was 1 ng of DNA template. A total of 230 commercial samples (160 fried and 70 processed fish products) were screened, where 60% mislabeling in fried and 30% mislabeling in processed fish were found. This multiplex PCR protocol could give useful insights for food inspection and enforcement of regulatory food control.

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检测水产品中错误标签的多重 PCR 分析法。

水产品中的鱼类替代品是水产品市场的一个重要问题，因为这种做法非常普遍。利用多重 PCR 技术开发了罗非鱼、泰拉鱼和罗非鱼的鉴定方案。利用线粒体基因组的 Cytb 基因设计了三个物种特异性引物和一个退化的通用正向引物。这些引物在琼脂糖凝胶上的片段大小分别为 235 bp、186 bp 和 506 bp。鲈鱼和鲶鱼的引物对 0.1 ng DNA 模板敏感，而黑鲈的引物对 1 ng DNA 模板敏感。共筛查了 230 份商业样品（160 份油炸鱼和 70 份加工鱼产品），发现油炸鱼和加工鱼分别有 60% 和 30% 的错误标记。该多重 PCR 方案可为食品检测和食品监管执法提供有用的信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food additives & contaminants. Part B, Surveillance 农林科学-毒理学

CiteScore

5.30

自引率

10.30%

发文量

审稿时长

2.7 months

期刊介绍： Food Additives & Contaminants: Part B publishes surveillance data indicating the presence and levels of occurrence of designated food additives, residues and contaminants in foods, food supplements and animal feed. Data using validated methods must meet stipulated quality standards to be acceptable and must be presented in a prescribed format for subsequent data-handling. Food Additives & Contaminants: Part B restricts its scope to include certain classes of food additives, residues and contaminants. This is based on a goal of covering those areas where there is a need to record surveillance data for the purposes of exposure and risk assessment. The scope is initially restricted to: Additives - food colours, artificial sweeteners, and preservatives; Residues – veterinary drug and pesticide residues; Contaminants – metals, mycotoxins, phycotoxins, plant toxins, nitrate/nitrite, PCDDs/PCFDs, PCBs, PAHs, acrylamide, 3-MPCD and contaminants derived from food packaging. Readership: The readership includes scientists involved in all aspects of food safety and quality and particularly those involved in monitoring human exposure to chemicals from the diet. Papers reporting surveillance data in areas other than the above should be submitted to Part A . The scope of Part B will be expanded from time-to-time to ensure inclusion of new areas of concern.