A comparative study of tissue protein synthesis rates in an Antarctic, Harpagifer antarcticus and a temperate, Lipophrys pholis teleost

IF 2.1 3区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Keiron P.P. Fraser , Lloyd S. Peck , Melody S. Clark , Andrew Clarke
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Abstract

The affect of temperature on tissue protein synthesis rates has been reported in temperate and tropical, but not Antarctic fishes. Previous studies have generally demonstrated low growth rates in Antarctic fish species in comparison to temperate relatives and elevated levels of protein turnover. This study investigates how low temperatures effect tissue protein synthesis and hence tissue growth in a polar fish species. Groups of Antarctic, Harpagifer antarcticus and temperate, Lipophrys pholis, were acclimated to a range of overlapping water temperatures and protein synthesis was measure in white muscle (WM), liver and gastrointestinal tract (GIT). WM protein synthesis rates increased linearly with temperature in both species (H. antarcticus 0.16–0.23%.d−1, L. pholis, 0.31–0.76%.d−1), while liver (H. antarcticus 0.24–0.27%.d−1, L. pholis, 0.44–1.03%.d−1) and GIT were unaffected by temperature in H. antarcticus but increased non-linearly in L. pholis (H. antarcticus 0.22–0.26%.d−1, L. pholis, 0.40–0.86%.d−1). RNA to protein ratios were unaffected by temperature in H. antarcticus but increased weakly, in L. pholis WM and liver. In L. pholis, RNA translational efficiency increased significantly with temperature in all tissues, but only in liver in H. antarcticus. At the overlapping temperature of 3 °C, protein synthesis (WM 26%, Liver, 39%, GIT, 35%) and RNA translational efficiency (WM 273%, Liver, 271%, GIT, 300%) were significantly lower in H. antarcticus than L. pholis, while RNA to protein ratios were significantly higher (WM 270%, Liver 170%, GIT 186%). Tissue specific effects of temperature are detectable in both species. This study provides the first evidence, that tissue protein synthesis rates are constrained in Antarctic fishes.

Abstract Image

对南极鱼(Harpagifer antarcticus)和温带鱼(Lipophrys pholis teleost)组织蛋白质合成率的比较研究。
温度对组织蛋白质合成率的影响在温带和热带鱼类中有报道,但在南极鱼类中没有。以往的研究普遍表明,与温带鱼类相比,南极鱼类的生长率较低,蛋白质周转水平较高。本研究调查了低温如何影响极地鱼类的组织蛋白质合成,进而影响组织生长。将一组南极鱼(Harpagifer antarcticus)和温带鱼(Lipophrys pholis)置于一系列重叠的水温下,测量白肌(WM)、肝脏和胃肠道(GIT)的蛋白质合成。两个物种的白肌蛋白质合成率均随温度的升高而线性增加(H. antarcticus 0.16-0.23%.d-1,L. pholis 0.31-0.76%.d-1),而肝脏(H. antarcticus 0.24-0.27%.d-1,L. pholis 0.44-1%.d-1)的蛋白质合成率则随温度的升高而线性增加。pholis,0.44-1.03%.d-1)和消化道不受温度影响,但在 L. pholis 中则非线性增加(H. antarcticus 0.22-0.26%.d-1,L. pholis,0.40-0.86%.d-1)。在 H. antarcticus 中,RNA 与蛋白质的比率不受温度影响,但在 L. pholis 的 WM 和肝脏中,RNA 与蛋白质的比率增加较弱。在 L. pholis 的所有组织中,RNA 的翻译效率随温度的升高而显著增加,但在 H. antarcticus 中,只有肝脏的翻译效率随温度的升高而显著增加。在 3 °C 的重叠温度下,南极海蛇的蛋白质合成(WM 26%,肝脏 39%,胃肠道 35%)和 RNA 翻译效率(WM 273%,肝脏 271%,胃肠道 300%)明显低于北极海蛇,而 RNA 与蛋白质的比率则明显高于北极海蛇(WM 270%,肝脏 170%,胃肠道 186%)。在这两个物种中都能检测到温度对特定组织的影响。这项研究首次证明南极鱼类的组织蛋白质合成率受到限制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
5.00
自引率
4.30%
发文量
155
审稿时长
3 months
期刊介绍: Part A: Molecular & Integrative Physiology of Comparative Biochemistry and Physiology. This journal covers molecular, cellular, integrative, and ecological physiology. Topics include bioenergetics, circulation, development, excretion, ion regulation, endocrinology, neurobiology, nutrition, respiration, and thermal biology. Study on regulatory mechanisms at any level of organization such as signal transduction and cellular interaction and control of behavior are also published.
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