Ghizlane Krif, Rachid Lahlali, Aicha El Aissami, Salah-Eddine Laasli, Abdelaziz Mimouni, Abdelfattah A. Dababat, Btissam Zoubi, Fouad Mokrini
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引用次数: 0
Abstract
The root-knot nematode (RKN) Meloidogyne javanica is one of the most economically important plant pathogens. Due to the high toxicity of chemical nematicides, the use of nematophagous fungi represents a promising alternative in nematode management. These biological agents offer a more sustainable and environmentally friendly approach to controlling plant parasitic nematodes (PPNs). The present study aimed to isolate and evaluate the nematicidal activity of three nematophagous fungi, viz., Paraconiothyrium cyclothyrioides, Aspergillus oryzae, and Lecanicillium psalliotae, against M. javanica under laboratory and greenhouse conditions. Three densities (1 × 106, 1 × 107, and 1 × 108 spores/ml) of each fungus were used. In vitro, the results revealed that all the tested isolates were effective at inhibiting egg hatching and mortality in second juveniles (J2s). However, the mortality of J2s and hatching inhibition of eggs were proportional to the fungal concentration and duration of the exposure period. Among the isolates, A. oryzae at a concentration of 1 × 108 spores/ml had the highest percentage of egg-hatching inhibition (95.5%) after 72 h of incubation. The highest juvenile mortality (100%) was recorded for P. cyclothyrioides at 1 × 108 spores/ml after 72 h. The in vivo results showed that all the tested isolates significantly reduced the number of galls, egg masses, and eggs in tomato roots and J2s in soil. In general, the greatest reductions in the galling index (40%) and number of egg masses per root (88.8%) were recorded for P. cyclothyrioides at 1 × 108 spores/ml, while the greatest reductions in the numbers of eggs (96.7) and J2s (98.1) were recorded for A. oryzae at 1 × 108 spores/ml. Moreover, the treatment of soil with P. cyclothyrioides, A. oryzae, L. psalliotae, and NemGuard granules resulted in significant increases in root and shoot length; in contrast, a decrease in root fresh weight was observed. Therefore, these data suggest that the three isolates, viz., P. cyclothyrioides, A. oryzae, and L. psalliotae, are essential elements for integrated M. javanica control in tomato crops.
期刊介绍:
Gesunde Pflanzen publiziert praxisbezogene Beiträge zum Pflanzenschutz in Landwirtschaft, Forstwirtschaft, Gartenbau und öffentlichem Grün und seinen Bezügen zum Umwelt- und Verbraucherschutz sowie zu Rechtsfragen.
Das Themenspektrum reicht von der Bestimmung der Schadorganismen über Maßnahmen und Verfahren zur Minderung des Befallsrisikos bis hin zur Entwicklung und Anwendung nicht-chemischer und chemischer Bekämpfungsstrategien und -verfahren, aber auch zu Fragen der Auswirkungen des Pflanzenschutzes auf die Umwelt, die Sicherung der Ernährung sowie zu allgemeinen Fragen wie Nutzen und Risiken und zur Entwicklung neuer Technologien.
Jedes Heft enthält Originalbeiträge renommierter Wissenschaftler, aktuelle Informationen von Verbänden sowie aus der Industrie, Pressemitteilungen und Personalia.
Damit bietet die Zeitschrift vor allem Behörden und Anwendern im Agrarsektor und Verbraucherschutz fundierte Praxisunterstützung auf wissenschaftlichem Niveau.