Evgeniya A. Tikhonova, Pavel G. Georgiev, Oksana G. Maksimenko
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引用次数: 0
Abstract
Dosage compensation complex (DCC), which consists of five proteins and two non-coding RNAs roX, specifically binds to the X chromosome in males, providing a higher level of gene expression necessary to compensate for the monosomy of the sex chromosome in male Drosophila compared to the two X chromosomes in females. The MSL2 protein contains the N-terminal RING domain, which acts as an E3 ligase in ubiquitination of proteins and is the only subunit of the complex expressed only in males. Functional role of the two C-terminal domains of the MSL2 protein, enriched with proline (P-domain) and basic amino acids (B-domain), was investigated. As a result, it was shown that the B-domain destabilizes the MSL2 protein, which is associated with the presence of two lysines ubiquitination of which is under control of the RING domain of MSL2. The unstructured proline-rich domain stimulates transcription of the roX2 gene, which is necessary for effective formation of the dosage compensation complex.
摘要剂量补偿复合体（DCC）由5个蛋白质和2个非编码RNA roX组成，特异性地与雄性果蝇的X染色体结合，与雌性果蝇的两条X染色体相比，DCC提供了更高水平的基因表达，以补偿雄性果蝇性染色体的单体性。MSL2 蛋白含有 N 端 RING 结构域，该结构域在蛋白质泛素化过程中起 E3 连接酶的作用，是该复合体中仅在雄性果蝇中表达的唯一亚基。研究人员对富含脯氨酸（P-domain）和碱性氨基酸（B-domain）的 MSL2 蛋白的两个 C 端结构域的功能作用进行了研究。结果表明，B-结构域会破坏 MSL2 蛋白的稳定性，这与 MSL2 的 RING 结构域控制两个赖氨酸泛素化有关。非结构化富脯氨酸结构域刺激了roX2基因的转录，而roX2基因是有效形成剂量补偿复合物的必要条件。
期刊介绍：
Biochemistry (Moscow) is the journal that includes research papers in all fields of biochemistry as well as biochemical aspects of molecular biology, bioorganic chemistry, microbiology, immunology, physiology, and biomedical sciences. Coverage also extends to new experimental methods in biochemistry, theoretical contributions of biochemical importance, reviews of contemporary biochemical topics, and mini-reviews (News in Biochemistry).
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