In vitro and in silico studies of the acaricidal and anticholinesterase activities of Randia aculeata seeds against the southern cattle tick Rhipicephalus (Boophilus) microplus.

IF 1.3 4区 农林科学 Q2 Veterinary
Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-04-29 eCollection Date: 2024-01-01 DOI:10.1590/S1984-29612024021
Aarón Salvador Bustos-Baena, José Luis Bravo-Ramos, Dora Romero-Salas, Sokani Sánchez-Montes, Luis Arturo Ortiz-Carbajal, María Guadalupe Sánchez-Otero
{"title":"In vitro and in silico studies of the acaricidal and anticholinesterase activities of Randia aculeata seeds against the southern cattle tick Rhipicephalus (Boophilus) microplus.","authors":"Aarón Salvador Bustos-Baena, José Luis Bravo-Ramos, Dora Romero-Salas, Sokani Sánchez-Montes, Luis Arturo Ortiz-Carbajal, María Guadalupe Sánchez-Otero","doi":"10.1590/S1984-29612024021","DOIUrl":null,"url":null,"abstract":"<p><p>Rhipicephalus (Boophilus) microplus is a leading cause of significant economic losses in the livestock industry, and tick populations have developed multiple forms of resistance to acaricides; therefore, the potential of novel natural bioactive compounds that are effective for targeting ticks must be addressed. The aim of this study was to evaluate the acaricidal and anticholinesterase activities of R. aculeata seeds and to identify naturally occurring compounds that potentially inhibit anticholinesterase through in silico docking. The acaricidal activity of the extract of R. aculeata seeds against larval and adult R. microplus ticks was assessed through immersion tests. Inhibition of anticholinesterase activity was measured spectrophotometrically. Extracts of R. aculeata seeds showed activity against larvae and engorged females of R. microplus, and a reduction in the reproductive index were also observed. Rutin, chlorogenic acid, quercetin, and epicatechin exhibited noteworthy interactions with the active site residues of RmAChE. These findings could significantly contribute to the exploration of novel natural products that can potentially inhibit RmAChE and could be used in the development of new acaricides for tick control.</p>","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 2","pages":"e001524"},"PeriodicalIF":1.3000,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11065400/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista Brasileira De Parasitologia Veterinaria","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1590/S1984-29612024021","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"Veterinary","Score":null,"Total":0}
引用次数: 0

Abstract

Rhipicephalus (Boophilus) microplus is a leading cause of significant economic losses in the livestock industry, and tick populations have developed multiple forms of resistance to acaricides; therefore, the potential of novel natural bioactive compounds that are effective for targeting ticks must be addressed. The aim of this study was to evaluate the acaricidal and anticholinesterase activities of R. aculeata seeds and to identify naturally occurring compounds that potentially inhibit anticholinesterase through in silico docking. The acaricidal activity of the extract of R. aculeata seeds against larval and adult R. microplus ticks was assessed through immersion tests. Inhibition of anticholinesterase activity was measured spectrophotometrically. Extracts of R. aculeata seeds showed activity against larvae and engorged females of R. microplus, and a reduction in the reproductive index were also observed. Rutin, chlorogenic acid, quercetin, and epicatechin exhibited noteworthy interactions with the active site residues of RmAChE. These findings could significantly contribute to the exploration of novel natural products that can potentially inhibit RmAChE and could be used in the development of new acaricides for tick control.

针对南方牛蜱 Rhipicephalus (Boophilus) microplus 的 Randia aculeata 种子杀螨和抗胆碱酯酶活性的体外和硅学研究。
Rhipicephalus (Boophilus) microplus 是造成畜牧业重大经济损失的主要原因,蜱虫种群已经对杀螨剂产生了多种形式的抗药性;因此,必须解决新型天然生物活性化合物的潜力问题,这些化合物可以有效地针对蜱虫。本研究的目的是评估 R. aculeata 种子的杀螨和抗胆碱酯酶活性,并通过硅对接找出可能抑制抗胆碱酯酶的天然化合物。通过浸泡试验评估了 R. aculeata 种子提取物对幼虫和成虫 R. microplus 蜱的杀螨活性。分光光度法测量了抗胆碱酯酶活性的抑制作用。R. aculeata 种子提取物对幼虫和吞食的雌性 R. microplus 具有活性,同时还观察到生殖指数的降低。芦丁、绿原酸、槲皮素和表儿茶素与 RmAChE 的活性位点残基有显著的相互作用。这些发现将极大地促进对可能抑制 RmAChE 的新型天然产品的探索,并可用于开发控制蜱虫的新型杀螨剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Revista Brasileira De Parasitologia Veterinaria
Revista Brasileira De Parasitologia Veterinaria PARASITOLOGY-VETERINARY SCIENCES
CiteScore
2.60
自引率
7.70%
发文量
90
审稿时长
>12 weeks
期刊介绍: La revista es un órgano de difusión del Colegio Brasileño de Parasitología Veterinaria, con una especificidad dentro de esa área, la difusión de los resultados de la investigación brasileña en las áreas de Helmintología, Protozoología, Entomología y agentes transmitidos por artrópodos, relacionados con la salud animal.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信