Trehalose improves the movement ability of Aβ_arcDrosophila by restoring the damaged mitochondria.

IF 1.8 4区医学 Q4 NEUROSCIENCES

Translational Neuroscience Pub Date : 2024-04-10 eCollection Date: 2024-01-01 DOI:10.1515/tnsci-2022-0338

Liangxian Li, Zhiheng Huang, Mingli Wu, Xia Li, Bo Xiao, Dong Yao, Biwen Mo

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引用次数: 0

Abstract

Background: The deposition of Aβ₄₂ has been regarded as one of the important pathological features of Alzheimer's disease (AD). However, drug development for Aβ₄₂ toxicity has been progressed slowly.

Objective: Our aim was to introduce the effect and related mechanism of trehalose on an Aβ_arc (arctic mutant Aβ₄₂) Drosophila AD model.

Methods: The human Aβ_arc was expressed in Drosophila to construct the AD model. Trehalose was added to the culture vial. The movement ability was determined by detecting climbing ability and flight ability. Enzyme-linked immunosorbent assay was used to detect the levels of Aβ_arc, ATP, and lactate. Electron microscopy assay, mitochondrial membrane potential assay, and mitochondrial respiration assay were used to assess the mitochondrial structure and function.

Results: Trehalose strongly improved the movement ability of Aβ_arc Drosophila in a concentration gradient-dependent manner. Furthermore, trehalose increased the content of ATP and decreased the content of Aβ_arc and lactate both in the brain and thorax of Aβ_arc Drosophila. More importantly, the mitochondrial structure and function were greatly improved by trehalose treatment in Aβ_arc Drosophila.

Conclusion: Trehalose improves movement ability at least partly by reducing the Aβ_arc level and restoring the mitochondrial structure and function in Aβ_arc Drosophila.

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通过恢复受损的线粒体，树胶糖提高了 AβarcDrosophila 的运动能力。

背景：Aβ42 的沉积一直被认为是阿尔茨海默病（AD）的重要病理特征之一。然而，针对 Aβ42 毒性的药物开发进展缓慢：我们的目的是介绍三卤糖对 Aβarc（北极突变体 Aβ42）果蝇 AD 模型的影响及相关机制：方法：在果蝇中表达人Aβarc，构建AD模型。方法：在果蝇体内表达人 Aβarc 以构建 AD 模型。通过检测爬行能力和飞行能力来确定果蝇的运动能力。酶联免疫吸附试验检测 Aβarc、ATP 和乳酸的水平。电镜检测、线粒体膜电位检测和线粒体呼吸检测用于评估线粒体结构和功能：结果：在浓度梯度依赖性作用下，曲哈洛糖能显著提高 Aβarc 果蝇的运动能力。此外，在 Aβarc 果蝇的大脑和胸部，三卤糖都能增加 ATP 的含量，降低 Aβarc 和乳酸的含量。更重要的是，经曲阿露糖处理的 Aβarc 果蝇的线粒体结构和功能得到了极大改善：结论：通过降低 Aβarc 水平和恢复 Aβarc 果蝇的线粒体结构和功能，树胶糖至少部分改善了果蝇的运动能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Translational Neuroscience NEUROSCIENCES-

CiteScore

3.00

自引率

4.80%

发文量

审稿时长

>12 weeks

期刊介绍： Translational Neuroscience provides a closer interaction between basic and clinical neuroscientists to expand understanding of brain structure, function and disease, and translate this knowledge into clinical applications and novel therapies of nervous system disorders.