The prognostic activity of acylglycerol kinase immunohistochemical expression in colon adenocarcinoma patients.

IF 1.7 Q3 GASTROENTEROLOGY & HEPATOLOGY

Przegla̜d Gastroenterologiczny Pub Date : 2023-01-01 Epub Date: 2023-12-08 DOI:10.5114/pg.2023.133477

Marlena Brzozowa-Zasada, Józef Kurek, Marek Kucharzewski, Katarzyna Stęplewska

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引用次数: 0

Abstract

Introduction: Adenocarcinoma of the colon and rectum (COAD) is one of the most commonly diagnosed cancers of the gastrointestinal system. Acylglycerol kinase (AGK) is a known lipid kinase producing lysophosphatidic acid (LPA) from monoacylglycerol. It is widely expressed in the heart, brain, kidney, and muscle. Moreover, AGK is a significant cancer-related gene and is upregulated in many human malignancies, e.g. prostate cancer, breast cancer, oral squamous cell carcinoma, hepatocellular carcinoma, and renal carcinoma. However, the expression pattern and clinical significance of AGK in colon adenocarcinoma patients, especially in individuals living in Europe, remain unclear.

Aim: The current study investigated the expression of AGK protein in colon adenocarcinoma samples to assess its prognostic significance by correlating its immunohistochemical expression with the clinicopathological variables and survival of individuals living in Poland.

Material and methods: Tissue specimens were received from 110 colon adenocarcinoma patients who underwent surgical resection at the Municipal Hospital in Jaworzno in 2013-2015. The paraffin-embedded specimens were cut into 4-µm-thick sections and incubated with rabbit polyclonal antibody to AGK (final dilution 1 : 500) (Invitrogen; cat. number PA5-28566).

Results and conclusions: AGK was strongly expressed in colon adenocarcinoma tissues in comparison to non-pathological colon specimens. The high level of AGK immunoexpression was demonstrated to be clearly correlated with the malignancy-related clinicopathological factors and 5-year overall survival rate of patients.

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结肠腺癌患者中酰基甘油激酶免疫组化表达的预后活性。

简介结肠和直肠腺癌（COAD）是最常见的消化系统癌症之一。甘油酰激酶（AGK）是一种由单酰基甘油产生溶血磷脂酸（LPA）的已知脂质激酶。它在心脏、大脑、肾脏和肌肉中广泛表达。此外，AGK 还是一种重要的癌症相关基因，在前列腺癌、乳腺癌、口腔鳞状细胞癌、肝细胞癌和肾癌等多种人类恶性肿瘤中都会出现上调。目的：本研究调查了结肠腺癌样本中 AGK 蛋白的表达情况，通过将其免疫组化表达与临床病理变量和波兰居民的生存率相关联，评估其预后意义：组织标本来自 2013-2015 年在 Jaworzno 市立医院接受手术切除的 110 名结肠腺癌患者。石蜡包埋的标本被切成 4 微米厚的切片，并与 AGK 兔多克隆抗体（最终稀释度 1 : 500）（Invitrogen 公司；商品编号 PA5-28566）一起孵育：与非病理结肠标本相比，AGK在结肠腺癌组织中强表达。AGK的高水平免疫表达与恶性肿瘤相关的临床病理因素和患者的5年总生存率明显相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Przegla̜d Gastroenterologiczny GASTROENTEROLOGY & HEPATOLOGY-

CiteScore

2.20

自引率

7.70%

发文量

审稿时长

6-12 weeks

期刊介绍： Gastroenterology Review is a journal published each 2 months, aimed at gastroenterologists and general practitioners. Published under the patronage of Consultant in Gastroenterology and Polish Pancreatic Club.