Metabolomic profiling of embryo culture media in patients with repeated implantation failure during assisted reproductive technology cycles.

IF 1.8 Q3 OBSTETRICS & GYNECOLOGY
Sheyda Nami, Azam Govahi, Nabaa Najjar, Samaneh Ghasemi, Farshid Rezaei, Fatemehsadat Amjadi, Gholamreza Taheripak
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Abstract

Objective: This study investigated the metabolic status of the spent culture media from embryos of patients with repeated implantation failure (RIF) undergoing in vitro fertilization-intracytoplasmic sperm injection cycles in comparison with the embryos from healthy fertile women.

Methods: Metabolite levels in spent culture media were assessed and compared between embryos from RIF patients (n=35) and oocyte donors as controls (n=15). Protein levels of insulin-like growth factor 1 (IGF-1) were determined using Western blotting. Concentrations of glucose, pyruvate, and lactate were measured using spectrophotometry. Ionic colorimetric assay kits were utilized to analyze the concentrations of sodium, chloride, calcium, and magnesium ions. High-performance liquid chromatography was employed to measure the concentrations of glutamic acid, aspartic acid, methionine, phenylalanine, and histidine.

Results: Glucose consumption and lactate secretion were higher in the control group than in the RIF group. The magnesium concentration was significantly higher in the control group than in the RIF group, but glutamic acid and aspartic acid concentrations were lower in the control group than in the RIF patients (p<0.05). The levels of IGF-1, sodium, calcium, chloride, methionine, histidine, and phenylalanine did not show statistically significant differences between the two groups.

Conclusion: The metabolic profile of the culture medium of the embryos in the RIF group differed from that of the control group. These findings suggest potential factors that may affect implantation capacity in RIF patients and provide a new perspective on embryo selection.

辅助生殖技术周期中反复植入失败患者的胚胎培养基代谢组学分析。
研究目的本研究调查了体外受精-卵胞浆内单精子注射周期中反复植入失败(RIF)患者胚胎与健康育龄妇女胚胎废培养基的代谢状况:方法:评估用过的培养基中的代谢物水平,并将 RIF 患者的胚胎(35 个)与作为对照的卵母细胞捐献者的胚胎(15 个)进行比较。用 Western 印迹法测定胰岛素样生长因子 1 (IGF-1) 蛋白水平。使用分光光度法测量葡萄糖、丙酮酸和乳酸的浓度。离子比色分析试剂盒用于分析钠离子、氯离子、钙离子和镁离子的浓度。采用高效液相色谱法测量谷氨酸、天冬氨酸、蛋氨酸、苯丙氨酸和组氨酸的浓度:结果:对照组的葡萄糖消耗量和乳酸分泌量均高于 RIF 组。对照组的镁浓度明显高于 RIF 组,但对照组的谷氨酸和天冬氨酸浓度低于 RIF 患者(p):RIF组胚胎培养基的代谢情况与对照组不同。这些发现提示了可能影响 RIF 患者植入能力的潜在因素,并为胚胎选择提供了新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
3.30
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