Functional characterization of follicle-stimulating hormone and luteinizing hormone receptors in cloudy catshark, Scyliorhinus torazame

IF 2.1 3区医学 Q3 ENDOCRINOLOGY & METABOLISM

General and comparative endocrinology Pub Date : 2024-04-27 DOI:10.1016/j.ygcen.2024.114542

Shogo Arimura , Marty Kwok Shing Wong , Ryotaro Inoue , Mai Kawano , Koya Shimoyama , Chika Fujimori , Kotaro Tokunaga , Wataru Takagi , Susumu Hyodo

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Abstract

The follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) in cloudy catshark were cloned, and recombinant FSHR and LHR were expressed for characterization. Ventral lobe extract (VLE) from the pituitary contains homologous FSH and LH, and it stimulated the cAMP signaling of FSHR and LHR dose-dependently. Two transcript variants of LHR (LHR-L with exon 10 and LHR-S without) were identified, and LHR-S was the dominant form with higher basal cAMP activity without VLE stimulation. Among various developmental stages of follicles, FSHR expression was mainly associated with the pre-vitellogenic and early white follicles. When follicles were recruited into vitellogenesis, the expression of FSHR decreased while of LHR was upregulated reciprocally, suggesting that LHR may also be responsible for the control of vitellogenesis in chondrichthyans. The expression of LHR-L was upregulated among maturing follicles before ovulation, indicating LHR-L could have a specific role in receiving the LH surge signal for final maturation. Plasma LH-like activity was transiently increased prior to the progesterone (P4)-surge and testosterone-drop at the beginning of P4-phase, supporting a pituitary control of follicle-maturation via LH signaling in chondrichthyans. The expression of follicular LHR was downregulated during the P4-phase when LH-like activity was high, indicating that the LH-dependent downregulation of LHR is conserved in chondrichthyans as it is in other vertebrate lineages. (213 words)

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云纹猫鲨卵泡刺激素和黄体生成素受体的功能特征。

克隆了云纹猫鼬的卵泡刺激素受体（FSHR）和黄体生成素受体（LHR），并表达了重组的FSHR和LHR。垂体腹叶提取物（VLE）含有同源的FSH和LH，可剂量依赖性地刺激FSHR和LHR的cAMP信号转导。研究发现了两种LHR转录本变体（带外显子10的LHR-L和不带外显子10的LHR-S），其中LHR-S是显性变体，在没有VLE刺激的情况下具有更高的基础cAMP活性。在卵泡的不同发育阶段中，FSHR的表达主要与卵泡生成前期和早期白卵泡有关。当卵泡进入卵黄发生期时，FSHR的表达下降，而LHR的表达则上调，这表明LHR可能也是控制软骨鱼类卵黄发生的因子。在排卵前，成熟卵泡中LHR-L的表达上调，表明LHR-L可能在接收LH激增信号以实现最终成熟方面发挥了特殊作用。血浆LH样活性在孕酮（P4）激增和P4期开始时睾酮下降之前短暂增加，支持软骨鱼类通过LH信号控制卵泡成熟。当LH样活性较高时，卵泡LHR的表达在P4期被下调，这表明LH依赖性的LHR下调在软骨鱼类中与在其他脊椎动物中一样是保守的。(213个字）。

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来源期刊

General and comparative endocrinology 医学-内分泌学与代谢

CiteScore

5.60

自引率

7.40%

发文量

120

审稿时长

2 months

期刊介绍： General and Comparative Endocrinology publishes articles concerned with the many complexities of vertebrate and invertebrate endocrine systems at the sub-molecular, molecular, cellular and organismal levels of analysis.