Ira Widjiastuti, Sri Kunarti, Evri Kusumah Ningtyas, Ayu Rafania Atikah, Khadijah Fauzi Basalamah, Amelia Evita Puspita, Novelia Ayu Widianti, Tengku Natasha Eleena Binti Tengku Ahmad Noor
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Abstract
Introduction: This study explored the effects of propolis extract calcium hydroxide (Ca(OH)2) combination on malondialdehyde and superoxide dismutase expression in dental pulp, aiming to assess its potential as a direct pulp capping material.
Materials and methods: Thirty male Wistar rats were randomly assigned to three groups. Mandibular molar teeth were prepared using a low-speed round bur. In Group I, no material was applied; in Group II, teeth were treated with Ca(OH)2; and in Group III, teeth were treated with Propolis extract-Ca(OH)2, followed by Cention N filling. Immunohistochemistry was conducted on pulp tissue samples obtained on the third and seventh days post-treatment to assess malondialdehyde and superoxide dismutase expression. Statistical analyses included the Shapiro-Wilk test, Levene test, ANOVA, and Tukey's HSD.
Results: The samples treated with propolis extract-Ca(OH)2 combination exhibited significantly lower malondialdehyde expression on both days compared to samples treated with Ca(OH)2 (P<0.05), indicating reduced oxidative stress. Superoxide dismutase expression in the propolis extract-Ca(OH)2 group was higher (P<0.05), suggesting an enhanced antioxidant activity. The control group showed intermediate results. Statistical analyses confirmed significant differences between groups for both malondialdehyde and superoxide dismutase expressions (P<0.05).
Conclusion: The study suggests that the propolis extract-Ca(OH)2 combination holds promise for direct pulp capping applications by minimizing oxidative stress and promoting antioxidant defense mechanisms in dental pulp.
期刊介绍：
The Iranian Endodontic Journal (IEJ) is an international peer-reviewed biomedical publication, the aim of which is to provide a scientific medium of communication for researchers throughout the globe. IEJ aims to publish the highest quality articles, both clinical and scientific, on all aspects of Endodontics. The journal is an official Journal of the Iranian Center for Endodontic Research (ICER) and the Iranian Association of Endodontists (IAE). The Journal welcomes articles related to the scientific or applied aspects of endodontics e.g. original researches, systematic reviews, meta-analyses, review articles, clinical trials, case series/reports, hypotheses, letters to the editor, etc. From the beginning (i.e. since 2006), the IEJ was the first open access endodontic journal in the world, which gave readers free and instant access to published articles and enabling them faster discovery of the latest endodontic research.
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