PD-BAT: A novel approach of pooling basophil donors for expansion of commercial laboratory testing of Chronic Spontaneous Urticaria

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of immunological methods Pub Date : 2024-04-27 DOI:10.1016/j.jim.2024.113679

Saintedym Wills , Jessica Chavez , Ajay Grover , Nathanael Beck , Michele Romano , Christina Bauer , Michael Gerspach , Michael Schneider , Andre Valcour

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引用次数: 0

Abstract

The type II autoimmune subtype of Chronic Spontaneous Urticaria (CSU) is characterized by the presence of IgG autoantibodies targeting IgE or the IgE high-affinity receptor (FcεRI) on mast cells and basophils. In evaluation of CSU patients, indirect basophil activation testing (BAT), has been utilized, involving the mixing of patient serum with heterologous peripheral blood donors, followed by flow cytometric assessment of basophil markers. However, the reliability of the indirect BAT results hinges on the quality of the donor basophils utilized. In this study, we introduce an innovative approach where multiple potential basophil donors undergo rigorous BAT characterization alongside control samples. By selecting and pooling donors with optimal performance, we significantly enhance the inter-assay reproducibility of the indirect BAT test.

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PD-BAT：一种汇集嗜碱性粒细胞供体以扩大慢性自发性荨麻疹商业实验室检测范围的新方法。

慢性自发性荨麻疹（CSU）的 II 型自身免疫亚型的特点是肥大细胞和嗜碱性粒细胞上存在针对 IgE 或 IgE 高亲和力受体（FcεRI）的 IgG 自身抗体。在对 CSU 患者进行评估时，采用了间接嗜碱性粒细胞活化检测（BAT），包括将患者血清与异源外周血供体混合，然后用流式细胞仪评估嗜碱性粒细胞标记物。然而，间接 BAT 结果的可靠性取决于所使用的供体嗜碱性粒细胞的质量。在本研究中，我们引入了一种创新方法，让多个潜在的嗜碱性粒细胞供体与对照样本一起接受严格的 BAT 鉴定。通过选择和汇集性能最佳的供体，我们大大提高了间接嗜碱性粒细胞检测的测定间重现性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of immunological methods 医学-免疫学

CiteScore

4.10

自引率

0.00%

发文量

120

审稿时长

3 months

期刊介绍： The Journal of Immunological Methods is devoted to covering techniques for: (1) Quantitating and detecting antibodies and/or antigens. (2) Purifying immunoglobulins, lymphokines and other molecules of the immune system. (3) Isolating antigens and other substances important in immunological processes. (4) Labelling antigens and antibodies. (5) Localizing antigens and/or antibodies in tissues and cells. (6) Detecting, and fractionating immunocompetent cells. (7) Assaying for cellular immunity. (8) Documenting cell-cell interactions. (9) Initiating immunity and unresponsiveness. (10) Transplanting tissues. (11) Studying items closely related to immunity such as complement, reticuloendothelial system and others. (12) Molecular techniques for studying immune cells and their receptors. (13) Imaging of the immune system. (14) Methods for production or their fragments in eukaryotic and prokaryotic cells. In addition the journal will publish articles on novel methods for analysing the organization, structure and expression of genes for immunologically important molecules such as immunoglobulins, T cell receptors and accessory molecules involved in antigen recognition, processing and presentation. Submitted full length manuscripts should describe new methods of broad applicability to immunology and not simply the application of an established method to a particular substance - although papers describing such applications may be considered for publication as a short Technical Note. Review articles will also be published by the Journal of Immunological Methods. In general these manuscripts are by solicitation however anyone interested in submitting a review can contact the Reviews Editor and provide an outline of the proposed review.