Stability Indicating Method Development and Validation of Glycyrrhizin Using RP-HPLC-DAD: Application to Glycyrrhiza glabra Extract.

IF 1.5 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of chromatographic science Pub Date : 2024-05-05 DOI:10.1093/chromsci/bmae022

Waibiangki Lyngdoh, Sandeep Jat, Pramod Kumar

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引用次数: 0

Abstract

Glycyrrhiza glabra is commonly known as licorice. Licorice is the major source of glycyrrhizin. There is no reported stability indicating method for glycyrrhizin in the literature so far. Therefore, it was proposed to develop a stability indicating method and validate the method for glycyrrhizin and its application in G. glabra root extract. Method validation parameters were performed as per the International Council for Harmonization guidelines. The chromatographic separation was achieved on a Zorbax Extended C-18 (250 × 4.6 mm, 5 μm) column. The separation achieved using the mobile phase consisted of 0.1% formic acid in water and acetonitrile in gradient elution. The flow rate was kept at 1 mL/min, and ultraviolet-visible spectroscopy detection was at 250 nm. The average retention time of glycyrrhizin was found to be 7.30 min. Stress degradation studies were performed and confirmed that only acidic degradation has shown a degradation profile of glycyrrhizin up to 40%. The percentage of glycyrrhizin was found to be 0.40% in the G. glabra extract. This may be further explored for commercial applications.

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利用 RP-HPLC-DAD 开发和验证甘草苷的稳定性指示方法：在甘草提取物中的应用

甘草俗称甘草。甘草是甘草苷的主要来源。迄今为止，文献中还没有关于甘草苷稳定性指示方法的报道。因此，建议开发一种稳定性指示方法，并验证该方法在甘草根提取物中的应用。方法验证参数按照国际协调理事会的指导原则执行。色谱分离采用 Zorbax Extended C-18 (250 × 4.6 mm, 5 μm)色谱柱。以 0.1% 甲酸水溶液和乙腈为流动相进行梯度洗脱。流速为 1 mL/min，紫外可见光谱检测波长为 250 nm。甘草苷的平均保留时间为 7.30 分钟。进行了应力降解研究，结果表明只有酸性降解才会使甘草苷的降解率达到 40%。研究发现，甘草提取物中甘草苷的比例为 0.40%。可进一步探索其商业应用。

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来源期刊

Journal of chromatographic science 化学-分析化学

CiteScore

2.90

自引率

7.70%

发文量

审稿时长

5.6 months

期刊介绍： The Journal of Chromatographic Science is devoted to the dissemination of information concerning all methods of chromatographic analysis. The standard manuscript is a description of recent original research that covers any or all phases of a specific separation problem, principle, or method. Manuscripts which have a high degree of novelty and fundamental significance to the field of separation science are particularly encouraged. It is expected the authors will clearly state in the Introduction how their method compares in some markedly new and improved way to previous published related methods. Analytical performance characteristics of new methods including sensitivity, tested limits of detection or quantification, accuracy, precision, and specificity should be provided. Manuscripts which describe a straightforward extension of a known analytical method or an application to a previously analyzed and/or uncomplicated sample matrix will not normally be reviewed favorably. Manuscripts in which mass spectrometry is the dominant analytical method and chromatography is of marked secondary importance may be declined.