Stability Indicating Method Development and Validation of Glycyrrhizin Using RP-HPLC-DAD: Application to Glycyrrhiza glabra Extract.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-05-05 DOI:10.1093/chromsci/bmae022

Waibiangki Lyngdoh, Sandeep Jat, Pramod Kumar

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引用次数: 0

Abstract

Glycyrrhiza glabra is commonly known as licorice. Licorice is the major source of glycyrrhizin. There is no reported stability indicating method for glycyrrhizin in the literature so far. Therefore, it was proposed to develop a stability indicating method and validate the method for glycyrrhizin and its application in G. glabra root extract. Method validation parameters were performed as per the International Council for Harmonization guidelines. The chromatographic separation was achieved on a Zorbax Extended C-18 (250 × 4.6 mm, 5 μm) column. The separation achieved using the mobile phase consisted of 0.1% formic acid in water and acetonitrile in gradient elution. The flow rate was kept at 1 mL/min, and ultraviolet-visible spectroscopy detection was at 250 nm. The average retention time of glycyrrhizin was found to be 7.30 min. Stress degradation studies were performed and confirmed that only acidic degradation has shown a degradation profile of glycyrrhizin up to 40%. The percentage of glycyrrhizin was found to be 0.40% in the G. glabra extract. This may be further explored for commercial applications.

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利用 RP-HPLC-DAD 开发和验证甘草苷的稳定性指示方法：在甘草提取物中的应用

甘草俗称甘草。甘草是甘草苷的主要来源。迄今为止，文献中还没有关于甘草苷稳定性指示方法的报道。因此，建议开发一种稳定性指示方法，并验证该方法在甘草根提取物中的应用。方法验证参数按照国际协调理事会的指导原则执行。色谱分离采用 Zorbax Extended C-18 (250 × 4.6 mm, 5 μm)色谱柱。以 0.1% 甲酸水溶液和乙腈为流动相进行梯度洗脱。流速为 1 mL/min，紫外可见光谱检测波长为 250 nm。甘草苷的平均保留时间为 7.30 分钟。进行了应力降解研究，结果表明只有酸性降解才会使甘草苷的降解率达到 40%。研究发现，甘草提取物中甘草苷的比例为 0.40%。可进一步探索其商业应用。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.