Evaluation of differences in expression pattern of three isoforms of the transforming growth factor beta in patients with lumbosacral stenosis.

IF 3.4 3区 生物学 Q3 CELL BIOLOGY
Cell Cycle Pub Date : 2024-03-01 Epub Date: 2024-05-02 DOI:10.1080/15384101.2024.2345484
Dawid Sobański, Paweł Bogdał, Rafał Staszkiewicz, Małgorzata Sobańska, Michał Filipowicz, Ryszard Adam Czepko, Damian Strojny, Beniamin Oskar Grabarek
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引用次数: 0

Abstract

The study investigates molecular changes in the lumbosacral (L/S) spine's yellow ligamentum flavum during degenerative stenosis, focusing on the role of transforming growth factor beta 1-3 (TGF-β-1-3). Sixty patients with degenerative stenosis and sixty control participants underwent molecular analysis using real-time quantitative reverse transcription reaction technique (RTqPCR), enzyme-linked immunosorbent assay (ELISA), Western blot, and immunohistochemical analysis (IHC). At the mRNA level, study samples showed reduced expression of TGF-β-1 and TGF-β-3, while TGF-β-2 increased by only 4%. Conversely, at the protein level, the study group exhibited significantly higher concentrations of TGF-β-1, TGF-β-2, and TGF-β-3 compared to controls. On the other hand, at the protein level, a statistically significant higher concentration of TGF-β-1 was observed (2139.33 pg/mL ± 2593.72 pg/mL vs. 252.45 pg/mL ± 83.89 pg/mL; p < 0.0001), TGF-β-2 (3104.34 pg/mL ± 1192.74 pg/mL vs. 258.86 pg/mL ± 82.98 pg/mL; p < 0.0001), TGF-β-3 (512.75 pg/mL ± 107.36 pg/mL vs. 55.06 pg/mL ± 9.83 pg/mL, p < 0.0001) in yellow ligaments obtained from patients of the study group compared to control samples. The study did not establish a significant correlation between TGF-β-1-3 concentrations and pain severity. The findings suggest that molecular therapy aimed at restoring the normal expression pattern of TGF-β-1-3 could be a promising strategy for treating degenerative stenosis of the L/S spine. The study underscores the potential therapeutic significance of addressing molecular changes at the TGF-β isoforms level for better understanding and managing degenerative spinal conditions.

评估腰骶椎管狭窄症患者体内转化生长因子 beta 三种同工酶表达模式的差异。
该研究调查了腰骶部(L/S)脊柱黄韧带在退行性狭窄过程中的分子变化,重点研究了转化生长因子β1-3(TGF-β-1-3)的作用。60 名退行性狭窄患者和 60 名对照组参与者通过实时定量反转录反应技术(RTqPCR)、酶联免疫吸附试验(ELISA)、Western 印迹和免疫组化分析(IHC)进行了分子分析。在 mRNA 水平上,研究样本显示 TGF-β-1 和 TGF-β-3 的表达量减少,而 TGF-β-2 仅增加了 4%。相反,在蛋白质水平上,研究组的 TGF-β-1、TGF-β-2 和 TGF-β-3 浓度明显高于对照组。另一方面,在蛋白质水平上,观察到 TGF-β-1 的浓度明显高于对照组(2139.33 pg/mL ± 2593.72 pg/mL vs. 252.45 pg/mL ± 83.89 pg/mL;p p p)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cell Cycle
Cell Cycle 生物-细胞生物学
CiteScore
7.70
自引率
2.30%
发文量
281
审稿时长
1 months
期刊介绍: Cell Cycle is a bi-weekly peer-reviewed journal of high priority research from all areas of cell biology. Cell Cycle covers all topics from yeast to man, from DNA to function, from development to aging, from stem cells to cell senescence, from metabolism to cell death, from cancer to neurobiology, from molecular biology to therapeutics. Our goal is fast publication of outstanding research.
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