Thioredoxin promotes the regeneration and binding of elastic fibre and basement membrane

IF 2.7 4区医学 Q2 DERMATOLOGY

International Journal of Cosmetic Science Pub Date : 2024-04-29 DOI:10.1111/ics.12964

Takeshi Tohgasaki, Shino Nishizawa, Xingyu Yu, Shinya Kondo, Shioji Ishiwatari

{"title":"Thioredoxin promotes the regeneration and binding of elastic fibre and basement membrane","authors":"Takeshi Tohgasaki, Shino Nishizawa, Xingyu Yu, Shinya Kondo, Shioji Ishiwatari","doi":"10.1111/ics.12964","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objective</h3>\n \n <p>Thioredoxin (TRX), a ubiquitous protein with strong antioxidant activity, decreases in the skin with age. A decrease in TRX is expected to induce cellular senescence, chronic inflammation, and degeneration and loss of extracellular matrix (ECM), such as collagen and elastin within the skin. In this study, we investigated the effects of TRX addition to excised skin or skin models to understand the role of TRX on cells and ECM within the skin.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>To evaluate its effect on skin cells, we cultured a three-dimensional (3D) skin model in a medium containing TRX. The mRNA expression levels of proteins related to elastic and collagen fibres and the basement membrane were determined. Furthermore, 3D imaging and computational analysis were performed to evaluate the effect of TRX on the elastic fibres and extending COL VII structures in excised human skin after coculturing with TRX for 1, 4, 5 and 6 days.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Thioredoxin application to a 3D skin model upregulated elastin, <i>COLI</i> and <i>COLVII</i> mRNA expression. Applying TRX to the excised skin increased the number of linear elastic fibres. This effect of TRX demonstrated a daily increment in a dose-dependent manner. Thioredoxin extended the fibrous structure of COL VII into the dermis, expanding its colocalization region with elastic fibres. These structural effects were confirmed using 3D imaging and computational methods.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>Thioredoxin elongates elastic fibres from the dermis to the basement membrane and extends the COL VII structure from the basement membrane to the dermis in excised human skin. These findings suggest the potential of TRX to protect the skin against age-related alterations such as wrinkles and sagging.</p>\n </section>\n </div>","PeriodicalId":13936,"journal":{"name":"International Journal of Cosmetic Science","volume":"46 5","pages":"786-794"},"PeriodicalIF":2.7000,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Cosmetic Science","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/ics.12964","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DERMATOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Objective

Thioredoxin (TRX), a ubiquitous protein with strong antioxidant activity, decreases in the skin with age. A decrease in TRX is expected to induce cellular senescence, chronic inflammation, and degeneration and loss of extracellular matrix (ECM), such as collagen and elastin within the skin. In this study, we investigated the effects of TRX addition to excised skin or skin models to understand the role of TRX on cells and ECM within the skin.

Methods

To evaluate its effect on skin cells, we cultured a three-dimensional (3D) skin model in a medium containing TRX. The mRNA expression levels of proteins related to elastic and collagen fibres and the basement membrane were determined. Furthermore, 3D imaging and computational analysis were performed to evaluate the effect of TRX on the elastic fibres and extending COL VII structures in excised human skin after coculturing with TRX for 1, 4, 5 and 6 days.

Results

Thioredoxin application to a 3D skin model upregulated elastin, COLI and COLVII mRNA expression. Applying TRX to the excised skin increased the number of linear elastic fibres. This effect of TRX demonstrated a daily increment in a dose-dependent manner. Thioredoxin extended the fibrous structure of COL VII into the dermis, expanding its colocalization region with elastic fibres. These structural effects were confirmed using 3D imaging and computational methods.

Conclusion

Thioredoxin elongates elastic fibres from the dermis to the basement membrane and extends the COL VII structure from the basement membrane to the dermis in excised human skin. These findings suggest the potential of TRX to protect the skin against age-related alterations such as wrinkles and sagging.

查看原文本刊更多论文

硫氧还蛋白促进弹性纤维和基底膜的再生和结合

目的硫氧还原蛋白（TRX）是一种普遍存在的蛋白质，具有很强的抗氧化活性，但随着年龄的增长，皮肤中的硫氧还原蛋白会减少。预计 TRX 的减少会诱发细胞衰老、慢性炎症、细胞外基质（ECM）（如皮肤中的胶原蛋白和弹性蛋白）的退化和损失。为了评估 TRX 对皮肤细胞的影响，我们在含有 TRX 的培养基中培养了三维（3D）皮肤模型。我们测定了弹性纤维、胶原纤维和基底膜相关蛋白质的 mRNA 表达水平。此外，还进行了三维成像和计算分析，以评估与 TRX 共同培养 1、4、5 和 6 天后，TRX 对弹性纤维和延伸的 COL VII 结构的影响。在切除的皮肤上涂抹 TRX 可增加线性弹性纤维的数量。TRX的这一效应表现为剂量依赖性的每日递增。硫氧还蛋白将 COL VII 的纤维结构延伸至真皮层，扩大了其与弹性纤维的共定位区域。结论在切除的人体皮肤中，硫氧还蛋白可将弹性纤维从真皮层延伸至基底膜，并将 COL VII 结构从基底膜延伸至真皮层。这些研究结果表明，TRX 有可能保护皮肤免受皱纹和松弛等与年龄有关的变化的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

International Journal of Cosmetic Science DERMATOLOGY-

CiteScore

4.60

自引率

4.30%

发文量

期刊介绍： The Journal publishes original refereed papers, review papers and correspondence in the fields of cosmetic research. It is read by practising cosmetic scientists and dermatologists, as well as specialists in more diverse disciplines that are developing new products which contact the skin, hair, nails or mucous membranes. The aim of the Journal is to present current scientific research, both pure and applied, in: cosmetics, toiletries, perfumery and allied fields. Areas that are of particular interest include: studies in skin physiology and interactions with cosmetic ingredients, innovation in claim substantiation methods (in silico, in vitro, ex vivo, in vivo), human and in vitro safety testing of cosmetic ingredients and products, physical chemistry and technology of emulsion and dispersed systems, theory and application of surfactants, new developments in olfactive research, aerosol technology and selected aspects of analytical chemistry.