Stable measurement of chemistry, immunochemistry, and hematology analytes in a heparin-based anticoagulant with iloprost additive: A promising candidate for the polyvalent blood collection tube

IF 2.2 4区医学 Q3 HEMATOLOGY

International Journal of Laboratory Hematology Pub Date : 2024-05-02 DOI:10.1111/ijlh.14291

Elias Frost Wiwe, Laura Emilie Schmidt, Emilie Zeuthen, Ida Maria Storm Engelstoft, Buris Brinch Christiansen, Thomas Steen Hansen, Melanie A. Burkhardt, Claus Antonio Juel Jensen

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引用次数: 0

Abstract

Introduction

A polyvalent blood collection tube could potentially reduce the number and volume of blood samples drawn from patients and reduce the risk of tube mix-ups in a point-of-care setting in the emergency department and the intensive care unit.

Methods

Four different concentrations of our experimental heparin anticoagulant with iloprost additive (HEP-ILOP 50 nM, 150 nM, 1000 nM, and 10 μM, respectively) were tested for significant differences and bias performance specifications against EDTA for 29 hematology analytes, and the highest concentration (HEP-ILOP 10 μM) against lithium heparin for 14 chemistry and immunochemistry analytes. Samples were drawn from 79 consenting subjects from the Oncology Department (n = 38) and the Intensive and Intermediary Care Unit (n = 41).

Results

For hematology analytes, the HEP-ILOP formulation generally provided stable measurement within optimal requirements within 5 h after sampling (mean 104 ± 56 min), with very little difference between the four HEP-ILOP concentrations. Because of differences in platelet and red blood cell swelling between EDTA and HEP-ILOP, all size-dependent analytes required proportional factorization to produce similar results. Platelet count by impedance similarly required factorization, whereas the fluorescent method provided results identical with EDTA. Chemistry and immunochemistry analytes were within optimal requirements except for potassium, lactate dehydrogenase, and glucose, indicating a cytoprotective effect of iloprost reducing cell metabolism and rupture, thereby producing results closer to in vivo conditions.

Conclusions

Our novel dry-sprayed anticoagulant formulation, HEP-ILOP, is a promising candidate for a polyvalent blood collection tube, enabling the analysis of hematology, chemistry, and immunochemistry analytes in the same tube.

Abstract Image

查看原文本刊更多论文

在以肝素为基础、添加伊洛前列素的抗凝剂中稳定测量化学、免疫化学和血液学分析物：多价采血管的理想候选产品

导言多价采血管有可能减少从患者身上抽取血液样本的数量和体积，并降低急诊科和重症监护室护理点环境中混管的风险。方法针对 29 种血液学分析物，测试了我们试验性肝素抗凝剂与伊洛前列素添加剂的四种不同浓度（HEP-ILOP 分别为 50 nM、150 nM、1000 nM 和 10 μM）与 EDTA 的显著差异和偏差性能指标；针对 14 种化学和免疫化学分析物，测试了最高浓度（HEP-ILOP 10 μM）与肝素锂的显著差异和偏差性能指标。结果对于血液学分析物，HEP-ILOP 配方通常在取样后 5 小时内（平均 104 ± 56 分钟）提供稳定的测量，符合最佳要求，四种 HEP-ILOP 浓度之间的差异很小。由于 EDTA 和 HEP-ILOP 在血小板和红细胞膨胀方面的差异，所有依赖于大小的分析物都需要进行比例因式分解才能得出相似的结果。通过阻抗法进行的血小板计数同样需要因式分解，而荧光法得出的结果与 EDTA 相同。除了钾、乳酸脱氢酶和葡萄糖之外，化学和免疫化学分析物都在最佳要求范围内，这表明伊洛前列素具有细胞保护作用，可减少细胞代谢和破裂，从而产生更接近体内条件的结果。结论我们的新型干喷抗凝剂配方 HEP-ILOP 是一种很有前途的多价采血管候选产品，可在同一采血管中分析血液学、化学和免疫化学分析物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Laboratory Hematology 医学-血液学

CiteScore

4.50

自引率

6.70%

发文量

211

审稿时长

6-12 weeks

期刊介绍： The International Journal of Laboratory Hematology provides a forum for the communication of new developments, research topics and the practice of laboratory haematology. The journal publishes invited reviews, full length original articles, and correspondence. The International Journal of Laboratory Hematology is the official journal of the International Society for Laboratory Hematology, which addresses the following sub-disciplines: cellular analysis, flow cytometry, haemostasis and thrombosis, molecular diagnostics, haematology informatics, haemoglobinopathies, point of care testing, standards and guidelines. The journal was launched in 2006 as the successor to Clinical and Laboratory Hematology, which was first published in 1979. An active and positive editorial policy ensures that work of a high scientific standard is reported, in order to bridge the gap between practical and academic aspects of laboratory haematology.