Zinc oxide and silver effects on the growth, pigment content and genetic stability of chrysanthemums propagated by the node culture method

IF 2.2 4区 农林科学 Q2 HORTICULTURE
Alicja Tymoszuk, Urszula Szałaj, Jacek Wojnarowicz, Jolanta Kowalska, Małgorzata Antkowiak, Dariusz Kulus
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引用次数: 0

Abstract

This article describes benefits of the application of zinc oxide submicron particles (ZnO SMPs), zinc oxide nanoparticles (ZnO NPs) and ZnO NPs combined with silver NPs (ZnO + Ag NPs) in chrysanthemum micropropagation. Single node explants of Chrysanthemum × morifolium (Ramat.) Hemsl. ‘UTP Burgundy Gold (UBG)’ and ‘UTP Pinky Gold (UPG)’ were inoculated on the Murashige and Skoog (MS) medium and treated with 100 mg · L−1, 200 mg · L−1, or 400 mg · L−1 ZnO SMPs, ZnO NPs (1.5% H2O), ZnO NPs (6% H2O), ZnO + 0.1% Ag NPs (1.5% H2O), ZnO + 0.1% Ag NPs (6% H2O), ZnO + 1% Ag NPs (1.5% H2O) and ZnO + 1% Ag NPs (6% H2O). Generally, the tested materials stimulated the growth and development of plantlets. In ‘UBG’, the most prominent treatments affecting increases in the number of leaves, micropropagation coefficient, shoot length and shoot FW/DW weight included 400 mg · L−1 ZnO SMPs and 100 mg · L−1 ZnO NPs (6% H2O). In ‘UPG’, the treatments with 200 mg · L−1 ZnO + 0.1% Ag NPs (6% H2O) and 200 mg · L−1 ZnO + 1% Ag NPs (6% H2O) were the most successful. The latter treatment stimulated an intensive development of root systems in the two studied cultivars. High values of leaf area, perimeter and width were reported in both cultivars for 400 mg · L−1 ZnO + 1% Ag NPs (6% H2O). As compared to the control, the treated plants were characterised by a similar or, most often, lower content of chlorophylls and carotenoids. The randomly amplified polymorphic DNA (RAPD) and start codon targeted polymorphism (SCoT) marker system analyses of the 400 mg · L−1 ZnO SMPs/ZnO NPs/ZnO + Ag NPs-treated chrysanthemums confirmed their genetic fidelity with the control plants. The obtained results can be implemented in the commercial large-scale production of chrysanthemums.
氧化锌和银对节点培养法繁殖菊花的生长、色素含量和遗传稳定性的影响
本文介绍了在菊花微繁殖中应用氧化锌亚微米颗粒(ZnO SMPs)、氧化锌纳米颗粒(ZnO NPs)以及氧化锌纳米颗粒与银纳米颗粒(ZnO + Ag NPs)的益处。菊花 × morifolium (Ramat.) Hemsl.将'UTP 勃艮第金(UBG)'和'UTP 粉红金(UPG)'接种到 Murashige and Skoog(MS)培养基上,用 100 mg - L-1、200 mg - L-1 或 400 mg - L-1 ZnO SMPs、ZnO NPs(1.5% H2O)、ZnO NPs(6% H2O)、ZnO + 0.1% Ag NPs(1.5% H2O)、ZnO + 0.1% Ag NPs(6% H2O)、ZnO + 1% Ag NPs(1.5% H2O)和 ZnO + 1% Ag NPs(6% H2O)处理。一般来说,测试材料都能刺激小植株的生长和发育。在 "UBG "中,对增加叶片数、微繁殖系数、芽长和芽全长/半径重量影响最大的处理包括 400 mg - L-1 ZnO SMPs 和 100 mg - L-1 ZnO NPs(6% H2O)。在 "UPG "中,200 mg - L-1 ZnO + 0.1% Ag NPs(6% H2O)和 200 mg - L-1 ZnO + 1% Ag NPs(6% H2O)的处理最为成功。后一种处理方法促进了两个研究品种根系的密集发展。400 mg - L-1 ZnO + 1% Ag NPs(6% H2O)处理的两个品种的叶面积、周长和宽度都很高。与对照组相比,处理过的植物叶绿素和类胡萝卜素含量相似,甚至更低。对 400 mg - L-1 ZnO SMPs/ZnO NPs/ZnO + Ag NPs 处理过的菊花进行的随机扩增多态性 DNA(RAPD)和起始密码子定向多态性(SCoT)标记系统分析证实了它们与对照植物的遗传保真度。所获得的结果可用于菊花的大规模商业化生产。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Folia Horticulturae
Folia Horticulturae Agricultural and Biological Sciences-Horticulture
CiteScore
3.40
自引率
0.00%
发文量
13
审稿时长
16 weeks
期刊介绍: Folia Horticulturae is an international, scientific journal published in English. It covers a broad research spectrum of aspects related to horticultural science that are of interest to a wide scientific community and have an impact on progress in both basic and applied research carried out with the use of horticultural crops and their products. The journal’s aim is to disseminate recent findings and serve as a forum for presenting views as well as for discussing important problems and prospects of modern horticulture, particularly in relation to sustainable production of high yield and quality of horticultural products, including their impact on human health.
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