Human umbilical cord-derived mesenchymal stem cells and auto-crosslinked hyaluronic acid gel complex for treatment of intrauterine adhesion

Jiaying Fan, Jingying Xie, Yunsheng Liao, Baoyu Lai, Guixin Zhou, Wenqin Lian, Jian Xiong
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Abstract

Objective: The purpose of this study was to explore the therapeutic characteristics of mesenchymal stem cells generated from human umbilical cord (hUC-MSCs) when utilized in conjunction with auto-crosslinked hyaluronic acid gel (HA-gel) for the management of intrauterine adhesion (IUA). The goal was to see how this novel therapy could enhance healing and improve outcomes for IUA patients. Methods: In this study, models of intrauterine adhesion (IUA) were established in Sprague-Dawley (SD) rats, which were then organized and divided into hUC-MSCs groups. The groups involved: hUC-MSCs/HA-gel group, control group, and HA-gel group. Following treatment, the researchers examined the uterine cavities and performed detailed analyses of the endometrial tissues to determine the effectiveness of the interventions. Results: The results indicated that in comparison with to the control group, both HA-gel, hUC-MSCs, and hUC-MSCs/HA-gel groups showed partial repair of IUA. However, in a more notable fashion transplantation of hUC-MSCs/HA-gel complex demonstrated significant dual repair effects. Significant outcomes were observed in the group treated with hUC-MSCs and HA-gel, they showed thicker endometrial layers, less fibrotic tissue, and a higher number of endometrial glands. This treatment strategy also resulted in a significant improvement in fertility restoration, indicating a profound therapeutic effect. Conclusions: The findings of this study suggest that both HA-gel, hUC-MSCs, and hUC-MSCs/HA-gel complexes have the potential for partial repair of IUA and fertility restoration caused by endometrium mechanical injury. Nonetheless, the transplantation of the hUC-MSCs/HA-gel complex displayed exceptional dual healing effects, combining effective anti-adhesive properties with endometrial regeneration stimuli.
用于治疗宫腔粘连的人脐带间充质干细胞和自交联透明质酸凝胶复合物
研究目的本研究的目的是探索人脐带间充质干细胞(hUC-MSCs)与自交联透明质酸凝胶(HA-gel)结合用于治疗宫腔内粘连(IUA)的治疗特性。我们的目标是了解这种新型疗法如何促进 IUA 患者的愈合并改善疗效。方法:本研究在斯普拉格-道利(SD)大鼠中建立了宫腔内粘连(IUA)模型,然后将其组织并分为 hUC-MSCs 组。各组包括:hUC-间充质干细胞/HA-凝胶组、对照组和HA-凝胶组。治疗后,研究人员检查了子宫腔,并对子宫内膜组织进行了详细分析,以确定干预措施的有效性。结果显示结果表明,与对照组相比,HA-凝胶组、hUC-间充质干细胞组和hUC-间充质干细胞/HA-凝胶组都显示出IUA的部分修复。然而,更值得注意的是,hUC-间充质干细胞/HA-凝胶复合物移植具有显著的双重修复效果。在使用 hUC-间充质干细胞和 HA 凝胶治疗的组别中观察到了显著的效果,它们显示出更厚的子宫内膜层、更少的纤维化组织和更多的子宫内膜腺体。这种治疗策略还显著改善了生育能力的恢复,显示出深远的治疗效果。结论:本研究结果表明,HA-凝胶、hUC-间充质干细胞和 hUC-间充质干细胞/HA-凝胶复合物都有可能部分修复子宫内膜机械损伤引起的 IUA 并恢复生育能力。然而,hUC-间充质干细胞/HA-凝胶复合物的移植显示出卓越的双重修复效果,将有效的抗粘连特性与子宫内膜再生刺激相结合。
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