{"title":"Formulation and Characterization of a Pluronic F127 Polymeric Micelle as a Nanocarrier for Berberine Delivery","authors":"N. Alyasari, A. Almzaiel","doi":"10.22146/ijc.88109","DOIUrl":null,"url":null,"abstract":"Berberine's (Ber’s) lower water solubility, which leads to low bioavailability, poses substantial delivery-related barriers to its therapeutic efficacy. Thus, a new approach to improving Ber's delivery and bioavailability is required. In this study, a Pluronic F127 micelle containing Ber (mBer) was formulated using thin-film hydration technique with the intention of resolving challenging issues associated with Ber delivery. The micelle was tested for drug loading and retention efficiency, size, zeta potential, shape, in vitro release, and in vitro toxicity. The spherical micelles that were made had an average encapsulation efficiency of 85%, a hydrodynamic size of 82.2 nm, a polydispersity of 0.176, and a zeta potential of −47.4 mV. The results of Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) indicated that Ber was physically entrapped and in an amorphous state within the synthesized micelles. Compared to the free Ber solution, the in vitro release of Ber from micelles exhibited both short-term rapid release and sustained release. The mBer was shown to be relatively non-toxic to blood cells via an in vitro hemolysis assay. Our findings showed that polymeric F127 micelles could be a simple nanocarrier for Ber delivery, which can be used to enhance the therapeutic efficiency of Ber.","PeriodicalId":13515,"journal":{"name":"Indonesian Journal of Chemistry","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indonesian Journal of Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22146/ijc.88109","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Berberine's (Ber’s) lower water solubility, which leads to low bioavailability, poses substantial delivery-related barriers to its therapeutic efficacy. Thus, a new approach to improving Ber's delivery and bioavailability is required. In this study, a Pluronic F127 micelle containing Ber (mBer) was formulated using thin-film hydration technique with the intention of resolving challenging issues associated with Ber delivery. The micelle was tested for drug loading and retention efficiency, size, zeta potential, shape, in vitro release, and in vitro toxicity. The spherical micelles that were made had an average encapsulation efficiency of 85%, a hydrodynamic size of 82.2 nm, a polydispersity of 0.176, and a zeta potential of −47.4 mV. The results of Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) indicated that Ber was physically entrapped and in an amorphous state within the synthesized micelles. Compared to the free Ber solution, the in vitro release of Ber from micelles exhibited both short-term rapid release and sustained release. The mBer was shown to be relatively non-toxic to blood cells via an in vitro hemolysis assay. Our findings showed that polymeric F127 micelles could be a simple nanocarrier for Ber delivery, which can be used to enhance the therapeutic efficiency of Ber.
小檗碱(Ber's)的水溶性较低,导致生物利用率较低,这对其治疗效果构成了很大的输送障碍。因此,需要一种新的方法来改善小檗碱的输送和生物利用率。本研究采用薄膜水合技术配制了含有 Ber 的 Pluronic F127 胶束(mBer),旨在解决与 Ber 给药相关的难题。研究人员对胶束的药物负载和保留效率、尺寸、ZETA电位、形状、体外释放和体外毒性进行了测试。制成的球形胶束的平均封装效率为 85%,流体力学尺寸为 82.2 nm,多分散性为 0.176,zeta 电位为 -47.4 mV。傅立叶变换红外光谱(FTIR)和 X 射线衍射(XRD)的结果表明,Ber 在合成胶束中处于物理夹带和无定形状态。与游离 Ber 溶液相比,胶束对 Ber 的体外释放表现出短期快速释放和持续释放两种特性。体外溶血试验表明,mBer 对血细胞相对无毒。我们的研究结果表明,聚合物 F127 胶束可以作为一种简单的纳米载体来递送 Ber,从而提高 Ber 的治疗效果。
期刊介绍:
Indonesian Journal of Chemistry is a peer-reviewed, open access journal that publishes original research articles, review articles, as well as short communication in all areas of chemistry, including educational chemistry, applied chemistry, and chemical engineering.