{"title":"Crafting Genetic Diversity: Unlocking the Potential of Protein Evolution","authors":"Vamsi Krishna Gali, K. L. Tee, T. S. Wong","doi":"10.3390/synbio2020009","DOIUrl":null,"url":null,"abstract":"Genetic diversity is the foundation of evolutionary resilience, adaptive potential, and the flourishing vitality of living organisms, serving as the cornerstone for robust ecosystems and the continuous evolution of life on Earth. The landscape of directed evolution, a powerful biotechnological tool inspired by natural evolutionary processes, has undergone a transformative shift propelled by innovative strategies for generating genetic diversity. This shift is fuelled by several factors, encompassing the utilization of advanced toolkits like CRISPR-Cas and base editors, the enhanced comprehension of biological mechanisms, cost-effective custom oligo pool synthesis, and the seamless integration of artificial intelligence and automation. This comprehensive review looks into the myriad of methodologies employed for constructing gene libraries, both in vitro and in vivo, categorized into three major classes: random mutagenesis, focused mutagenesis, and DNA recombination. The objectives of this review are threefold: firstly, to present a panoramic overview of recent advances in genetic diversity creation; secondly, to inspire novel ideas for further innovation in genetic diversity generation; and thirdly, to provide a valuable resource for individuals entering the field of directed evolution.","PeriodicalId":507619,"journal":{"name":"SynBio","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"SynBio","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/synbio2020009","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Genetic diversity is the foundation of evolutionary resilience, adaptive potential, and the flourishing vitality of living organisms, serving as the cornerstone for robust ecosystems and the continuous evolution of life on Earth. The landscape of directed evolution, a powerful biotechnological tool inspired by natural evolutionary processes, has undergone a transformative shift propelled by innovative strategies for generating genetic diversity. This shift is fuelled by several factors, encompassing the utilization of advanced toolkits like CRISPR-Cas and base editors, the enhanced comprehension of biological mechanisms, cost-effective custom oligo pool synthesis, and the seamless integration of artificial intelligence and automation. This comprehensive review looks into the myriad of methodologies employed for constructing gene libraries, both in vitro and in vivo, categorized into three major classes: random mutagenesis, focused mutagenesis, and DNA recombination. The objectives of this review are threefold: firstly, to present a panoramic overview of recent advances in genetic diversity creation; secondly, to inspire novel ideas for further innovation in genetic diversity generation; and thirdly, to provide a valuable resource for individuals entering the field of directed evolution.
遗传多样性是生物进化复原力、适应潜力和旺盛生命力的基础,是地球上强大生态系统和生命持续进化的基石。受自然进化过程的启发,定向进化这一强大的生物技术工具在基因多样性创新战略的推动下,发生了脱胎换骨的变化。推动这一转变的因素包括:CRISPR-Cas 和碱基编辑器等先进工具包的利用、对生物机制理解的提高、具有成本效益的定制寡核苷酸池合成,以及人工智能和自动化的无缝整合。这篇综述探讨了体外和体内构建基因文库的各种方法,主要分为三大类:随机诱变、聚焦诱变和 DNA 重组。这篇综述的目的有三:首先,对基因多样性创建的最新进展进行全景式概述;其次,启发进一步创新基因多样性生成的新思路;第三,为进入定向进化领域的个人提供有价值的资源。