A Genome-Wide Association Study of Seed Morphology-Related Traits in Sorghum Mini-Core and Senegalese Lines

Crops Pub Date : 2024-04-11 DOI:10.3390/crops4020012
E. Ahn, Sunchung Park, Zhenbin Hu, Vishnutej Ellur, Minhyeok Cha, Yoonjung Lee, L. Prom, Clint W. Magill
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引用次数: 1

Abstract

Sorghum (Sorghum bicolor L.) ranks fifth as the most crucial cereal crop globally, yet its seed morphology remains relatively unexplored. This study investigated seed morphology in sorghum based on 115 mini-core and 130 Senegalese germplasms. Eight seed morphology traits encompassing size, shape, and color parameters were assessed. Statistical analyses explored potential associations between these traits and resistance to three major sorghum diseases: anthracnose, head smut, and downy mildew. Furthermore, genome-wide association studies (GWAS) were conducted using phenotypic data from over 24,000 seeds and over 290,000 publicly available single nucleotide polymorphisms (SNPs) through the Genome Association and Prediction Integrated Tool (GAPIT) R package. Significant SNPs associated with various seed morphology traits were identified and mapped onto the reference sorghum genome to identify novel candidate defense genes.
高粱小核心品系和塞内加尔品系种子形态相关性状的全基因组关联研究
高粱(Sorghum bicolor L.)在全球最重要的谷类作物中排名第五,但其种子形态学却相对尚未得到研究。本研究以 115 个迷你核心种质资源和 130 个塞内加尔种质资源为基础,对高粱种子形态进行了研究。研究评估了八种种子形态特征,包括大小、形状和颜色参数。统计分析探讨了这些性状与高粱三种主要病害(炭疽病、头疫病和霜霉病)抗性之间的潜在关联。此外,通过基因组关联与预测集成工具(GAPIT)R软件包,利用超过24000颗种子的表型数据和超过29万个公开的单核苷酸多态性(SNPs)进行了全基因组关联研究(GWAS)。鉴定了与各种种子形态性状相关的重要 SNPs,并将其映射到参考高粱基因组上,从而鉴定出新的候选防御基因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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