Detection and High-Throughput Microbial Analysis of Particulate Matter in Houses and Downwind Areas of Duck Farms

Abstract

Particulate matter (PM) and the microorganisms of duck houses may have negative impacts on animal and human health. During 2021-2022, PM2.5 and PM10 inside and outside the duck house were sampled with a built-in air sampler in Tai’an City, Shandong Province, and the diversity and abundance of microorganisms within the PM were analyzed by macrogenomic and absolute sequence analysis. The results showed that PM2.5 and PM10 concentrations in the house and at downwind points exceeded the short-term (24 h) guideline of the global air quality guidelines (AQG). Macrogenome sequencing showed that the microbial composition of the PM2.5 samples was dominated by bacteria (exceed 85%); a total of 1316 bacterial genera and 110 fungal genera were identified in PM2.5 samples from duck house 1 in winter, which were much higher than the results of amplicon sequencing method reported before, and relatively high levels of the pathogenic bacteria (Coccidioides immitis, etc.) and the conditionally pathogenic bacterium (Rothia nasimurium) were identified at the species level. Absolute quantitative sequencing detected conditionally pathogenic bacteria and allergens at high levels in PM10 samples: Corynebacterium (5.6 × 10⁷ copies/g), Aerococcus (9.9 × 10⁶ copies/g), Alternaria (3.3 × 10⁶ copies/g), and Aspergillus (8.3 × 10⁵ copies/g). Moreover, Corynebacterium was the highest content of PM10 in summer and PM2.5 samples in winter, and its pathogenicity and potential threat should be noted. The diversity and relative abundance of microorganisms were similar in the duck house and at the downwind point. The results showed that the microorganisms in the house environment have a greater influence on the air environment around the downwind point and may pose a public health risk to the staff and the surrounding area.