Akkermansia muciniphila improves gastric cancer treatment by modulating the immune microenvironment.

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Future microbiology Pub Date : 2024-04-17 DOI:10.2217/fmb-2023-0210

Jia-hui Fang, Huizhong Zhang, Xiaodong Zhang, Xiaolong Lu, Junjie Liu, Haiyang Li, Jianxin Huang

引用次数: 0

Abstract

Background: Gut microbiota is pivotal in tumor occurrence and development, and there is a close relationship between Akkermansia muciniphila (AKK) and cancer immunotherapy. Methods: The effects of AKK and its outer membrane proteins on gastric cancer (GC) were evaluated in vitro and in vivo using cell counting kit-8 assay, flow cytometry, western blotting, ELISA, immunohistochemistry and immunofluorescence. Results: AKK outer membrane protein facilitated apoptosis of GC cells and exerted an immunostimulatory effect (by promoting M1 polarization of macrophages, enhancing expression of cytotoxic T-lymphocyte-related cytokines and suppressing that of Treg-related cytokines). Additionally, AKK and its formulation could inhibit tumor growth of GC and enhance the infiltration of immune cells in tumor tissues. Conclusion: AKK could improve GC treatment by modulating the immune microenvironment.

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Akkermansia muciniphila 可通过调节免疫微环境改善胃癌治疗。

背景：肠道微生物群在肿瘤的发生和发展过程中起着关键作用，而粘液蝇（Akkermansia muciniphila，AKK）与癌症免疫疗法之间有着密切的关系。研究方法采用细胞计数试剂盒-8测定法、流式细胞术、Western印迹、ELISA、免疫组织化学和免疫荧光等方法，在体外和体内评估了AKK及其外膜蛋白对胃癌（GC）的影响。结果AKK 外膜蛋白能促进 GC 细胞凋亡，并发挥免疫刺激作用（促进巨噬细胞的 M1 极化，增强细胞毒性 T 淋巴细胞相关细胞因子的表达，抑制 Treg 相关细胞因子的表达）。此外，AKK 及其制剂还能抑制 GC 肿瘤的生长，并增强肿瘤组织中免疫细胞的浸润。结论AKK 可通过调节免疫微环境改善 GC 治疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Future microbiology 生物-微生物学

CiteScore

4.90

自引率

3.20%

发文量

134

审稿时长

6-12 weeks

期刊介绍： Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.