Haematological and Biochemical Patterns in the Liver Function of a Syrup Made From Vitex Doniana Fruit

Abstract

BACKGROUND: Using an animal model, this study aimed to investigate the haematological and biochemical parameters of _Vitex doniana_ fruit syrup for its attribute to liver function. METHODOLOGY: Syrup concentrations of 25-45 mg/kgbw were considered on the mice with weights of 25-35 g. Groups of seven with 5 mice in each were conducted. Group 1 mice served as the negative control, and were given access to feed and water. Groups 2 – 6 mice were administered orally with 25, 30, 35, 40, and 45 ml of syrup concentrations in a single dose for 3 days. The automated haematologic analyser determined the haematology of the mice's blood. The in vivo antioxidants and biochemical assays were determined by chemical methods, and the histopathology with haematoxylin and eosin stain method. RESULTS: Weight gain of change values from 8.12-9.81 g was recorded. Increase in red blood cells (7.32–7.45 m/cu.mm), white blood cells (4.30–4.35 t/cu.mm), lymphocytes (55.21-54.72%), neutrophils (21.64–12.70%), and PCV (41.04–41.16% were recorded. Values of the catalase were 50.20-58.21 µM/g, glutathione (23.41-28.34 µM/g), and lipid peroxidation (93.54-106.21 µM/g) compared to the negative control that has values of between 48.37-58.27 µM/g). A decrease in unit value was recorded in the biomarker enzymes of alkaline phosphate that ranged from 91.17 to 85.24 IU/L, aspartate aminotransferase from 58.67 to 31.56 IU/L, and alanine aminotransferase from 58.16 to 43.36 IU/L. Decreased values in total bilirubin, creatinine, cholesterol, and urea were conceived compared with the negative control value. However, an increase in the unit was observed in the total albumin that ranged from 3.16 to 3.38 mg/dL), total protein from 6.42 to 6.74 mg/dL, and uric acid from 7.06 to 5.10 mg/dL) along with the syrup concentrations compared with the negative control of 3.75 mg/dL, 7.12 mg/dL, and 5.21 mg/dL respectively. CONCLUSION: The study concludes that the syrup has no health implications as it has the potential for blood maintenance, good antioxidant quality, and quality enzyme makers for healthy liver function.