Metagenomic next-generation sequencing for the diagnosis of neurobrucellosis.

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Future microbiology Pub Date : 2024-04-17 DOI:10.2217/fmb-2023-0177

Wen Li, Ying He, Yanyan Li, Xiaona Li, Ting Bian, Tingting Liu, Xuedong Liu, Wen Jiang

引用次数: 0

Abstract

Objective: This study investigates the application of metagenomic next-generation sequencing (mNGS) in the diagnosis of neurobrucellosis (NB). Methods: We retrospectively analyzed patients diagnosed with NB who underwent cerebrospinal fluid (CSF) mNGS testing in Xijing Hospital from 2015 to 2021. Results: Among the 20 individuals included in the study, the serum rose bengal test was positive in 11 out of 16 cases, serum agglutination test was positive in 13 out of 16 cases, CSF culture was positive in 6 out of 11 cases, and CSF mNGS tests were positive in 18 out of 20 cases. Conclusion: CSF mNGS demonstrates superior sensitivity; therefore, it is recommended to collect CSF for mNGS testing prior to antibiotic therapy when NB is suspected.

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用于诊断神经布鲁氏菌病的下一代元基因组测序。

研究目的本研究探讨了元基因组新一代测序（mNGS）在神经布鲁氏菌病（NB）诊断中的应用。方法我们回顾性分析了2015年至2021年在西京医院接受脑脊液（CSF）mNGS检测的确诊NB患者。结果在纳入研究的 20 例患者中，16 例中有 11 例血清玫瑰红试验呈阳性，16 例中有 13 例血清凝集试验呈阳性，11 例中有 6 例脑脊液培养呈阳性，20 例中有 18 例脑脊液 mNGS 试验呈阳性。结论：CSF mNGSCSF mNGS 具有更高的灵敏度；因此，建议在怀疑 NB 时，在抗生素治疗前采集 CSF 进行 mNGS 检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Future microbiology 生物-微生物学

CiteScore

4.90

自引率

3.20%

发文量

134

审稿时长

6-12 weeks

期刊介绍： Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.