Antibacterial activity of corydalis saxicola bunting total alkaloids against Porphyromonas gingivalis in vitro.

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Future microbiology Pub Date : 2024-04-17 DOI:10.2217/fmb-2023-0165

Wenli Ye, Jiaxuan Wu, Qiaozhi Jiang, Zhiheng Su, Haiqing Liao, Zhenmin Liu, Renchuan Tao, Xiangzhi Yong

引用次数: 0

Abstract

Aim: To investigate the antibacterial effects of Corydalis Saxicola bunting total alkaloid (CSBTA) on Porphyromonas gingivalis. Methods: SEM, chemical staining, RT-qPCR and ELISA were used to detect effects of CSBTA on P. gingivalis. Results: CSBTA treatment caused shrinkage and rupture of P. gingivalis morphology, decreased biofilm density and live bacteria in biofilm, as well as reduced mRNA expression of virulence genes hagA, hagB, kgp, rgpA and rgpB of P. gingivalis. Furthermore, NOK cells induced by CSBTA-treated P. gingivalis exhibited lower IL-6 and TNF-α expression levels. Conclusion: CSBTA is able to kill free P. gingivalis, disrupt the biofilm and weaken the pathogenicity of P. gingivalis. It has the potential to be developed as a drug against P. gingivalis infection.

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苣荬菜总生物碱对牙龈卟啉单胞菌的体外抗菌活性。

目的：研究刺五加鹀总生物碱（CSBTA）对牙龈卟啉单胞菌的抗菌作用。方法：扫描电镜、化学染色、RT采用扫描电镜、化学染色、RT-qPCR 和 ELISA 检测 CSBTA 对牙龈卟啉菌的影响。结果：CSBTACSBTA 处理会导致牙龈脓杆菌形态收缩和破裂，降低生物膜密度和生物膜中的活菌数，并减少牙龈脓杆菌毒力基因 hagA、haggB、kgp、rgpA 和 rgpB 的 mRNA 表达。此外，经 CSBTA 处理的牙龈脓疱诱导的 NOK 细胞的 IL-6 和 TNF-α 表达水平较低。结论CSBTA 能够杀死游离的牙龈脓胞，破坏生物膜并削弱牙龈脓胞的致病性。它有可能被开发成一种抗牙龈脓疱病感染的药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Future microbiology 生物-微生物学

CiteScore

4.90

自引率

3.20%

发文量

134

审稿时长

6-12 weeks

期刊介绍： Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.