AtMYB41 acts as a dual-function transcription factor that regulates the formation of lipids in an organ- and development-dependent manner

IF 4.2 3区 生物学 Q1 PLANT SCIENCES
Plant Biology Pub Date : 2024-04-18 DOI:10.1111/plb.13650
A. Keyl, V. Kwas, M. Lewandowska, C. Herrfurth, L. Kunst, I. Feussner
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Abstract

Abstract Image

AtMYB41 是一种双重功能转录因子,以器官和发育依赖的方式调节脂质的形成
植物角质层可控制非气孔失水,并可作为抵御生物因子的屏障,而异聚合物单宁及其相关蜡质则在特定的细胞壁位置组成性沉积。虽然已经确定了几种控制角质层形成的转录因子,但那些参与转录调控单宁生物合成的转录因子仍然特征不清。本研究的主要目的是进一步分析 R2R3-髓母细胞瘤(MYB)转录因子 AtMYB41 在植物整个发育过程中形成角质层、单宁和单宁相关蜡质的功能。为了进行功能分析,我们分析了 AtMYB41 的器官特异性表达模式,并利用 CRISPR/Cas9 系统生成了 Atmyb41ge 等位基因。研究了这些等位基因在胁迫下的根系生长和透水性。此外,还利用气相色谱法评估了不同器官的脂肪酸、蜡质、角质素和单宁酸组成。Atmyb41ge突变体的特征表明,AtMYB41分别负向调节叶片和种子中的角质脂类和脂肪酸的生物合成。值得注意的是,生化分析表明 AtMYB41 还能正向调节拟南芥茎中角质蜡的形成。总之,这些结果表明,AtMYB41 分别作为叶片和种子中角质层和脂肪酸生物合成的负调控因子,同时也是拟南芥茎中蜡生成的正调控因子。
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来源期刊
Plant Biology
Plant Biology 生物-植物科学
CiteScore
8.20
自引率
2.60%
发文量
109
审稿时长
3 months
期刊介绍: Plant Biology is an international journal of broad scope bringing together the different subdisciplines, such as physiology, molecular biology, cell biology, development, genetics, systematics, ecology, evolution, ecophysiology, plant-microbe interactions, and mycology. Plant Biology publishes original problem-oriented full-length research papers, short research papers, and review articles. Discussion of hot topics and provocative opinion articles are published under the heading Acute Views. From a multidisciplinary perspective, Plant Biology will provide a platform for publication, information and debate, encompassing all areas which fall within the scope of plant science.
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