Green synthesis and optimization of iron oxide nanoparticles using central composite design as MRI contrast agent

IF 1.8 4区材料科学 Q4 GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY

Green Materials Pub Date : 2024-04-07 DOI:10.1680/jgrma.23.00089

Yuwvaranni S, Amritta B, Weslen Vedakumari S, Chamundeeswari M

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引用次数: 0

Abstract

Iron nanoparticles (FeNPs) derived from herbal plants are being explored as potential MRI contrast agents due to their biocompatibility and ability for targeted drug delivery. We for the first time have attempted the usage of various novel combinations of leaf extracts like Azadirachta indica, and Piper betel and with two other Ayurvedic herbs extracts such as Asparagus racemosus and Symplocos for an ecofriendly green synthesis of FeNPs. The nanoparticles were coated on nanofibers made from banana pseudo (FENPs-NF) stem to enhance biocompatibility. The synthesis process was optimized using RSM and techniques like UV-Vis, VSM, AAS,FE-SEM, EDAX, and FT-IR was used for Characterization. Haemolytic, immune cell assays, MTT assay and MRI was analyzed. A peak at 325 nm through the UV-Vis spectroscopy, AAS, FE-SEM, EDAX, and FT-IR proved the presence of FeNPs. Haemolytic, immune cell assay was found to be compatible and MTT assay (MCF -7) showed inhibition at 250 µg/ml. The FeNPs and FeNPs-NF showed a good signal loss in MRI analysis at 1.5 Tesla based proving the property of contrast agent nature. Thus, synthesized FeNPs from herbal plant sources may open a new door for its potential application in the biomedical field as a chemical-free and biocompatible MRI contrast agent.

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利用中心复合设计绿色合成和优化作为磁共振成像造影剂的氧化铁纳米粒子

从草本植物中提取的铁纳米粒子（FeNPs）因其生物相容性和靶向给药能力，正被探索用作潜在的磁共振成像造影剂。我们首次尝试将 Azadirachta indica 和 Piper betel 等叶提取物与其他两种阿育吠陀草药提取物（如天门冬和 Symplocos）进行各种新型组合，以实现铁纳米粒子的生态友好型绿色合成。纳米粒子涂覆在由香蕉假茎（FENPs-NF）制成的纳米纤维上，以增强生物相容性。使用 RSM 对合成过程进行了优化，并使用 UV-Vis、VSM、AAS、FE-SEM、EDAX 和 FT-IR 等技术进行表征。对溶血、免疫细胞检测、MTT 检测和核磁共振成像进行了分析。通过紫外可见光谱、AAS、FE-SEM、EDAX 和傅立叶变换红外光谱，325 纳米处的峰值证明了铁纳米粒子的存在。溶血和免疫细胞检测结果表明它们是兼容的，MTT 检测（MCF -7）显示 250 µg/ml 的抑制作用。在 1.5 特斯拉磁共振成像分析中，FeNPs 和 FeNPs-NF 显示出良好的信号损失，证明了造影剂的性质。因此，从草本植物中合成的 FeNPs 可为其在生物医学领域的潜在应用打开一扇新的大门，成为一种不含化学物质且具有生物相容性的 MRI 造影剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Green Materials Environmental Science-Pollution

CiteScore

3.50

自引率

15.80%

发文量

期刊介绍： The focus of Green Materials relates to polymers and materials, with an emphasis on reducing the use of hazardous substances in the design, manufacture and application of products.