Phenolic Compound Content and Antioxidant Activity of Rheum ribes Shells

Abstract

The antioxidative and therapeutic properties of Rheum ribes, a plant indigenous to Turkey, have been extensively researched. However, little attention has been paid to compounds extracted from R. ribes shells. This study focused on assessing the phenolic compound content and antioxidative capabilities of R. ribes shells. We identified 44 out of 88 phytochemical compounds present in these shells using liquid chromatography-high-resolution mass spectrometry. Among these compounds, rutin hydrate M-OH₂, kuromanine, and procyanidin B2 emerged as the most abundant, whereas sinapic acid had the lowest concentration. Furthermore, antioxidant activity was determined using the 2,2-diphenyl-1-picrylhydrazyl assay, with the R. ribes shell extract exhibiting an activity level of 1.06 ± 0.3 mg Trolox equivalent/g of sample. In summary, this research explored the potential health advantages of R. ribes shells, thereby offering valuable insights for discovering novel bioactive compounds in natural resources for future drug development.