MdWRKY71 promotes the susceptibility of apple to Glomerella leaf spot by controlling salicylic acid degradation

IF 4.8 1区农林科学 Q1 PLANT SCIENCES

Molecular plant pathology Pub Date : 2024-04-15 DOI:10.1111/mpp.13457

Tingting Pei, Dongshan Niu, Yongxin Ma, Minghui Zhan, Jie Deng, Pengmin Li, Fengwang Ma, Changhai Liu

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Abstract

Glomerella leaf spot (GLS), a fungal disease caused by Colletotrichum fructicola, severely affects apple (Malus domestica) quality and yield. In this study, we found that the transcription factor MdWRKY71 was significantly induced by C. fructicola infection in the GLS‐susceptible apple cultivar Royal Gala. The overexpression of MdWRKY71 in apple leaves resulted in increased susceptibility to C. fructicola, whereas RNA interference of MdWRKY71 in leaves showed the opposite phenotypes. These findings suggest that MdWRKY71 functions as a susceptibility factor for the apple—C. fructicola interaction. Furthermore, MdWRKY71 directly bound to the promoter of the salicylic acid (SA) degradation gene Downy Mildew Resistant 6 (DMR6)‐Like Oxygenase 1 (DLO1) and promoted its expression, resulting in a reduced SA level. The sensitivity of 35S:MdWRKY71 leaves to C. fructicola can be effectively alleviated by knocking down MdDLO1 expression, confirming the critical role of MdWRKY71‐mediated SA degradation via regulating MdDLO1 expression in GLS susceptibility. In summary, we identified a GLS susceptibility factor, MdWRKY71, that targets the apple SA degradation pathway to promote fungal infection.

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MdWRKY71 通过控制水杨酸降解促进苹果对禾本科叶斑病的敏感性

由果孢子菌（Colletotrichum fructicola）引起的真菌病害--苹果叶斑病（GLS）严重影响苹果（Malus domestica）的品质和产量。在这项研究中，我们发现在易感 GLS 的苹果栽培品种 Royal Gala 中，果孢子菌感染会显著诱导转录因子 MdWRKY71。在苹果叶片中过表达 MdWRKY71 会增加对果孢子菌的易感性，而 RNA 干扰叶片中的 MdWRKY71 则显示出相反的表型。这些发现表明，MdWRKY71 是苹果与果蝇相互作用的易感因子。此外，MdWRKY71 直接与水杨酸（SA）降解基因抗霜霉病 6（DMR6）-Like 氧化酶 1（DLO1）的启动子结合并促进其表达，导致 SA 水平降低。通过敲低 MdDLO1 的表达，可以有效缓解 35S:MdWRKY71 叶片对 C. fructicola 的敏感性，证实了 MdWRKY71 通过调控 MdDLO1 的表达介导的 SA 降解在 GLS 易感性中的关键作用。总之，我们发现了一种 GLS 易感因子 MdWRKY71，它能靶向苹果 SA 降解途径促进真菌感染。

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来源期刊

Molecular plant pathology 生物-植物科学

CiteScore

9.40

自引率

4.10%

发文量

120

审稿时长

6-12 weeks

期刊介绍： Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.