Molecular evidence for the presence of a G7 genotype of Echinococcus granulosus in Slovakia

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-04-12 DOI:10.1017/s0022149x00700873

V. Šnábel, S. D'Amelio, K. Mathiopoulos, L. Turčeková, P. Dubinský

引用次数: 0

Abstract

Variability in Echinococcus granulosus is very important epidemiologically since strain characteristics may influence local patterns of transmission of hydatid disease. To classify the genotype presented in pig protoscoleces of the Slovak territory, a DNA-based approach has been used. Nucleotide sequences for a 471 bp region of the mitochondrial NADH dehydrogenase 1 (ND1) gene revealed a substantial affinity of isolates examined to the G7 genotype. Only a 0.9–3.4% sequence variation was recorded for E. granulosus samples compared with the reference G7 variant. To distinguish between G7 and G9 genotypes not differing in ND1 sequences, isolates were additionally examined by PCR-RFLP analysis of the nuclear ITS1 region. The resulting two-banded pattern is characteristic for the G7 strain. The data presented thus provides the first explicit evidence of the G7 genotype in the Slovak region.

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斯洛伐克存在颗粒棘球蚴 G7 基因型的分子证据

肉毒棘球蚴的变异在流行病学上非常重要，因为菌株特征可能会影响包虫病在当地的传播模式。为了对斯洛伐克境内猪原虫的基因型进行分类，我们采用了一种基于 DNA 的方法。线粒体 NADH 脱氢酶 1 (ND1) 基因 471 bp 区域的核苷酸序列显示，受检的分离株与 G7 基因型有很大的亲和力。与参考的 G7 变异型相比，E. granulosus 样本的序列变异仅为 0.9-3.4%。为了区分在 ND1 序列上没有差异的 G7 和 G9 基因型，还对分离物进行了核 ITS1 区域的 PCR-RFLP 分析。结果显示，双带模式是 G7 菌株的特征。因此，所提供的数据首次明确证明了斯洛伐克地区的 G7 基因型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.