Uridine diphosphate glucosyltransferase is vital for fenpropathrin resistance in Bombyx mori (Lepidoptera)

IF 2.3 2区农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Insect Molecular Biology Pub Date : 2024-04-13 DOI:10.1111/imb.12912

Kai-yi Zheng, Xiao-ying Zhang, Fasihul Lisan, Wen-Qin Lai, Qiang Zhang, Jun-li Lv, Zhan-peng Lu, Sheng Qin, Xia Sun, Shang-zhi Zhang, Xue-yang Wang, Li-shang Dai, Mu-wang Li

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Abstract

The silkworm (Bombyx mori) is an important model lepidopteran insect and can be used to identify pesticide resistance-related genes of great significance for biological control of pests. Uridine diphosphate glucosyltransferases (UGTs), found in all organisms, are the main secondary enzymes involved in the metabolism of heterologous substances. However, it remains uncertain if silkworm resistance to fenpropathrin involves UGT. This study observes significant variations in BmUGT expression among B. mori strains with variable fenpropathrin resistance post-feeding, indicating BmUGT's role in fenpropathrin detoxification. Knockdown of BmUGT with RNA interference and overexpression of BmUGT significantly decreased and increased BmN cell activity, respectively, indicating that BmUGT plays an important role in the resistance of silkworms to fenpropathrin. In addition, fenpropathrin residues were significantly reduced after incubation for 12 h with different concentrations of a recombinant BmUGT fusion protein. Finally, we verified the conservation of UGT to detoxify fenpropathrin in Spodoptera exigua: Its resistance to fenpropathrin decreased significantly after knocking down SeUGT. In a word, UGT plays an important role in silkworm resistance to fenpropathrin by directly degrading the compound, a function seen across other insects. The results of this study are of great significance for breeding silkworm varieties with high resistance and for biological control of pests.

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二磷酸尿苷葡萄糖基转移酶对昆虫（鳞翅目）的苯丙菊酯抗性至关重要

家蚕（Bombyx mori）是一种重要的鳞翅目昆虫模型，可用于鉴定与农药抗性相关的基因，对害虫的生物防治具有重要意义。二磷酸尿苷葡萄糖转移酶（UGTs）存在于所有生物体内，是参与异源物质代谢的主要次级酶。然而，家蚕对苯丙菊酯的抗性是否涉及 UGT 仍不确定。本研究观察到，在喂养后对甲氰菊酯产生不同抗性的家蚕品系中，BmUGT 的表达量存在显著差异，这表明 BmUGT 在甲氰菊酯解毒过程中发挥作用。用 RNA 干扰敲除 BmUGT 和过表达 BmUGT 分别显著降低和提高了 BmN 细胞的活性，表明 BmUGT 在家蚕对苯丙菊酯的抗性中起着重要作用。此外，用不同浓度的重组 BmUGT 融合蛋白培养 12 小时后，苯丙菊酯残留量明显减少。最后，我们验证了 UGT 在鞘翅目昆虫中对甲氰菊酯的解毒作用：在敲除 SeUGT 后，鞘翅目昆虫对甲氰菊酯的抗性明显降低。总之，UGT 通过直接降解苯丙菊酯化合物，在家蚕对苯丙菊酯的抗性中发挥了重要作用，这一功能在其他昆虫中也可见到。该研究结果对培育高抗性家蚕品种和害虫的生物防治具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Insect Molecular Biology 生物-昆虫学

CiteScore

4.80

自引率

3.80%

发文量

审稿时长

6-12 weeks

期刊介绍： Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).