Comparative evaluation of three real-time polymerase chain reaction assays to detect Myxobolus cerebralis

IF 1.5 4区农林科学 Q3 FISHERIES

Journal of aquatic animal health Pub Date : 2024-04-15 DOI:10.1002/aah.10220

Wade Cavender, Christine Swan, Skylar Wolf, Danielle Van Vliet, Alison Aceves Johnson, Anna Forest, Robert Shields, Thomas Loch, Christopher Knupp, John Drennan, Gavin Glenney, Sascha L. Hallett, Joe Marcino, Aimee Reed

引用次数: 0

Abstract

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对三种实时聚合酶链式反应测定法检测大脑粘孢子菌的比较评估

我们试图通过使用巢式聚合酶链反应（nPCR）和以前建立的三种实时定量 PCR（qPCR）检测方法，评估对旋毛虫病病原体 Myxobolus cerebralis（Mc）检测的准确性和可重复性：K18S（凯利 18S）、C18S（卡文德 18S）和 Hsp70（热休克蛋白 70）。我们采用 "适合目的 "的方法，结合实验室内部和实验室之间的测试，确定了一种分子检测方法，该方法与目前公认的 Mc.

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来源期刊

Journal of aquatic animal health 农林科学-兽医学

CiteScore

2.50

自引率

0.00%

发文量

审稿时长

>24 weeks

期刊介绍： The Journal of Aquatic Animal Health serves the international community of scientists and culturists concerned with the health of aquatic organisms. It carries research papers on the causes, effects, treatments, and prevention of diseases of marine and freshwater organisms, particularly fish and shellfish. In addition, it contains papers that describe biochemical and physiological investigations into fish health that relate to assessing the impacts of both environmental and pathogenic features.