Destruction of Biofilms of Gram-Positive and Gram-Negative Bacteria by Serine Protease PAPC from Aspergillus ochraceus

IF 1.3 4区生物学 Q4 MICROBIOLOGY

Microbiology Pub Date : 2024-04-01 DOI:10.1134/s0026261723604281

引用次数: 0

Abstract

Infections associated with biofilm formation by gram-positive and gram-negative microorganisms cause difficulty in therapy and are prone to transition into chronic forms. Approaches to degradation of the biofilm matrix are therefore in demand. In the present work, we show that recombinant PAPC serine protease from Aspergillus ochraceus were able to degrade mature biofilms formed by a number of gram-positive and gram-negative bacteria by 15‒20% at 50 µg/mL. At 100 µg/mL, the biomass of S. aureus and P. aeruginosa biofilms decreased by 50%. Thus, the PAPC may be a promising agent for biofilm removal and enhance the efficiency of antimicrobial therapy.

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赭曲霉丝氨酸蛋白酶 PAPC 对革兰氏阳性和革兰氏阴性菌生物膜的破坏作用

摘要与革兰氏阳性和革兰氏阴性微生物形成的生物膜有关的感染给治疗带来困难，并容易转变为慢性感染。因此，我们需要降解生物膜基质的方法。在本研究中，我们发现来自赭曲霉的重组 PAPC 丝氨酸蛋白酶在 50 µg/mL 的浓度下能降解一些革兰氏阳性和革兰氏阴性细菌形成的成熟生物膜 15-20%。当浓度为 100 µg/mL 时，金黄色葡萄球菌和绿脓杆菌生物膜的生物量减少 50%。因此，PAPC 可能是一种很有前景的生物膜清除剂，并能提高抗菌治疗的效率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbiology 生物-微生物学

CiteScore

2.40

自引率

13.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Microbiology is an is an international peer reviewed journal that covers a wide range of problems in the areas of fundamental and applied microbiology. The journal publishes experimental and theoretical papers, reviews on modern trends in different fields of microbiological science, and short communications with descriptions of unusual observations. The journal welcomes manuscripts from all countries in the English or Russian language.