Hydrophilic Interaction Liquid Chromatography for the Analysis of Pharmaceutical Formulations

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-03-08 DOI:10.2174/0115734110290557240305045032

Aleksandra Radoičić, Sandra Šegan, Aleksandra Dramićanin, Dušanka Milojković-Opsenica

引用次数: 0

Abstract

For a long time, Reversed-Phase Liquid Chromatography (RPLC) was the most dominant technique for the analysis of pharmaceutical compounds, but with poor efficiency in the separation of small polar molecules. From the efforts to solve the problem of insufficient retention of these molecules, during the last decades, a mode of liquid chromatography named Hydrophilic Interaction Liquid Chromatography (HILIC) has experienced vast expansion. It is based on the use of a highly hydrophilic stationary phase along with an aqueous mobile phase with high organic modifier content. In this review, the characteristics of stationary and mobile phases used in HILIC are described, and corresponding separation mechanisms are discussed. An overview of recently published papers dealing with the application of HILIC in analyzing pharmaceuticals in biological and non-biological samples is provided. Besides, the application of HILIC systems in the determination of the physicochemical properties of compounds is described.

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用于分析药物制剂的亲水相互作用液相色谱法

长期以来，反相液相色谱法（RPLC）是分析药物化合物的最主要技术，但其分离小极性分子的效率较低。为了解决这些分子保留不充分的问题，近几十年来，一种名为亲水作用液相色谱（HILIC）的液相色谱模式得到了广泛推广。它的基础是使用高亲水性固定相和有机改性剂含量高的水性流动相。本综述介绍了 HILIC 中使用的固定相和流动相的特点，并讨论了相应的分离机制。综述了近期发表的有关 HILIC 在生物和非生物样品中药物分析应用的论文。此外，还介绍了 HILIC 系统在化合物理化性质测定中的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.