LncRNA LINC01278 regulates the prognosis and related mechanisms of gastric cancer by targeting miR-129-5p

IF 2.1 4区医学 Q3 TOXICOLOGY

Journal of Environmental Pathology Toxicology and Oncology Pub Date : 2024-04-01 DOI:10.1615/jenvironpatholtoxicoloncol.2024053208

Zhenhua Wang, Zhengliang Li, Xiaojing Liu, Jundong Wang, Jiaxi Wang, Guoxiang Jiang, Haizhou Yu

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引用次数: 0

Abstract

Gastric cancer, a prevalent malady within the digestive tract, has a complex pathological mechanism and numerous patients. The regulation of gastric cancer process by long non-coding RNA (lncRNA) has laid a new exploration idea for the study of its molecular mechanism and the treatment of patients. The abnormal expressed genes in gastric cancer were screened by GSE193109 dataset. The correlation between LINC01278 and the likelihood of survival in patients suffering from gastric cancer was investigated by Kaplan-Meier survival curve and multivariate Cox analysis. LINC01278 in gastric cancer tissue samples and cells was verified via RT-qPCR. The CCK-8 and Transwell assay were selected to detect the growth activity of gastric cancer cells. The association between LINC01278 and miR-129-5p was validated through luciferase reporter assay and RNA-binding protein immunoprecipitation (RIP) assay. Correlation analysis of clinical features revealed an association between LINC01278 and the prognosis in gastric cancer patients. LINC01278 was actively expressed in gastric cancer, which exerts a tumor-promoting effect. Silencing LINC01278 suppressed the biological function of tumor cells through spongiform miR-129-5p. LINC01278 has the potential to serve as a novel biomarker, offering new avenues of research for the prognosis and treatment of gastric cancer.

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LncRNA LINC01278通过靶向miR-129-5p调控胃癌的预后及相关机制

胃癌是消化道内的高发恶性肿瘤，病理机制复杂，患者众多。长非编码 RNA（lncRNA）对胃癌发生过程的调控为研究其分子机制和治疗胃癌患者提供了新的探索思路。通过GSE193109数据集筛选了胃癌中异常表达的基因。通过Kaplan-Meier生存曲线和多变量Cox分析研究了LINC01278与胃癌患者生存可能性的相关性。通过 RT-qPCR 验证了胃癌组织样本和细胞中的 LINC01278。CCK-8和Transwell试验被用来检测胃癌细胞的生长活性。通过荧光素酶报告实验和 RNA 结合蛋白免疫沉淀（RIP）实验验证了 LINC01278 与 miR-129-5p 之间的关联。临床特征的相关分析表明，LINC01278与胃癌患者的预后有关。LINC01278在胃癌中表达活跃，具有促癌作用。沉默LINC01278可通过海绵状miR-129-5p抑制肿瘤细胞的生物学功能。LINC01278有望成为一种新型生物标记物，为胃癌的预后和治疗提供新的研究途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Environmental Pathology Toxicology and Oncology 医学-毒理学

CiteScore

3.80

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Journal of Environmental Pathology, Toxicology and Oncology publishes original research and reviews of factors and conditions that affect human and animal carcinogensis. Scientists in various fields of biological research, such as toxicologists, chemists, immunologists, pharmacologists, oncologists, pneumologists, and industrial technologists, will find this journal useful in their research on the interface between the environment, humans, and animals.