Binbin Si , Yang Yang , Muhammad Naveed , Fenghuan Wang , Malik Wajid Hussain Chan
{"title":"Characterizations of biogenic selenium nanoparticles and their anti-biofilm potential against Streptococcus mutans ATCC 25175","authors":"Binbin Si , Yang Yang , Muhammad Naveed , Fenghuan Wang , Malik Wajid Hussain Chan","doi":"10.1016/j.jtemb.2024.127448","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><p><em>S. mutans</em> has been identified as the primary pathogenic bacterium in biofilm-mediated dental caries. The biogenic selenium nanoparticles (SeNPs) produced by <em>L. plantarum</em> KNF-5 were used in this study against <em>S. mutans</em> ATCC 25175.</p></div><div><h3>Objectives</h3><p>The aims of this study were: <strong>(1)</strong> the biosynthesis of SeNPs by <em>L. plantarum</em> KNF-5, <strong>(2)</strong> the characterization of SeNPs, <strong>(3)</strong> the investigation of the inhibitory effect of biogenic SeNPs against <em>S. mutans</em> ATCC 25175, and (<strong>4)</strong> the determination of the anti-biofilm potential of SeNPS against <em>S. mutans</em> ATCC 25175.</p></div><div><h3>Methodology</h3><p>3 mL of the culture was added to 100 mL of MRS medium and incubated. After 4 h, Na<sub>2</sub>SeO<sub>3</sub> solution (concentration 100 μg/mL) was added and incubated at 37 °C for 36 h. The color of the culture solution changed from brownish-yellow to reddish, indicating the formation of SeNPs. The characterization of SeNPs was confirmed by UV-Vis spectrophotometry, FTIR, SEM-EDS and a particle size analyzer. The antibacterial activity was determined by the disk diffusion method, the MIC by the micro-double dilution method, and the biofilm inhibitory potential by the crystal violet method and the MTT assay. The effect of SeNPs on <em>S. mutans</em> ATCC 25175 was determined using SEM and CLSM spectrometry techniques. The sulfate-anthrone method was used to analyze the effect of SeNPs on insoluble extracellular polysaccharides. The expression of genes in <em>S. mutans</em> ATCC 25175 was analyzed by real-time quantitative polymerase chain reaction (RT-qPCR).</p></div><div><h3>Preparation of nanoparticles</h3><p>SeNPs produced by probiotic bacteria are considered a safe method. In this study, <em>L. plantarum</em> KNF-5 (probiotic strain) was used for the production of SeNPs.</p></div><div><h3>Results</h3><p>The biogenic SeNPs were spherical and coated with proteins and polysaccharides and had a diameter of about 270 nm. The MIC of the SeNPs against <em>S. mutans</em> ATCC 25175 was 3.125 mg/mL. Biofilm growth was also significantly suppressed at this concentration. The expression of genes responsible for biofilm formation (GtfB, GtfC, BrpA and GbpB,) was reduced when <em>S. mutans</em> ATCC 25175 was treated with SeNPs.</p></div><div><h3>Conclusion</h3><p>It was concluded that the biogenic SeNPs produced by <em>L. plantarum</em> KNF-5 was highly effective to inhibit the growth of <em>S. mutans</em> ATCC 25175.</p></div><div><h3>Novelty statement</h3><p>The application of biogenic SeNPs, a natural anti-biofilm agent against <em>S. mutans</em> ATCC 25175. In the future, this study will provide a new option for the prevention and treatment of dental caries.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":3.6000,"publicationDate":"2024-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Trace Elements in Medicine and Biology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0946672X24000683","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Introduction
S. mutans has been identified as the primary pathogenic bacterium in biofilm-mediated dental caries. The biogenic selenium nanoparticles (SeNPs) produced by L. plantarum KNF-5 were used in this study against S. mutans ATCC 25175.
Objectives
The aims of this study were: (1) the biosynthesis of SeNPs by L. plantarum KNF-5, (2) the characterization of SeNPs, (3) the investigation of the inhibitory effect of biogenic SeNPs against S. mutans ATCC 25175, and (4) the determination of the anti-biofilm potential of SeNPS against S. mutans ATCC 25175.
Methodology
3 mL of the culture was added to 100 mL of MRS medium and incubated. After 4 h, Na2SeO3 solution (concentration 100 μg/mL) was added and incubated at 37 °C for 36 h. The color of the culture solution changed from brownish-yellow to reddish, indicating the formation of SeNPs. The characterization of SeNPs was confirmed by UV-Vis spectrophotometry, FTIR, SEM-EDS and a particle size analyzer. The antibacterial activity was determined by the disk diffusion method, the MIC by the micro-double dilution method, and the biofilm inhibitory potential by the crystal violet method and the MTT assay. The effect of SeNPs on S. mutans ATCC 25175 was determined using SEM and CLSM spectrometry techniques. The sulfate-anthrone method was used to analyze the effect of SeNPs on insoluble extracellular polysaccharides. The expression of genes in S. mutans ATCC 25175 was analyzed by real-time quantitative polymerase chain reaction (RT-qPCR).
Preparation of nanoparticles
SeNPs produced by probiotic bacteria are considered a safe method. In this study, L. plantarum KNF-5 (probiotic strain) was used for the production of SeNPs.
Results
The biogenic SeNPs were spherical and coated with proteins and polysaccharides and had a diameter of about 270 nm. The MIC of the SeNPs against S. mutans ATCC 25175 was 3.125 mg/mL. Biofilm growth was also significantly suppressed at this concentration. The expression of genes responsible for biofilm formation (GtfB, GtfC, BrpA and GbpB,) was reduced when S. mutans ATCC 25175 was treated with SeNPs.
Conclusion
It was concluded that the biogenic SeNPs produced by L. plantarum KNF-5 was highly effective to inhibit the growth of S. mutans ATCC 25175.
Novelty statement
The application of biogenic SeNPs, a natural anti-biofilm agent against S. mutans ATCC 25175. In the future, this study will provide a new option for the prevention and treatment of dental caries.
期刊介绍:
The journal provides the reader with a thorough description of theoretical and applied aspects of trace elements in medicine and biology and is devoted to the advancement of scientific knowledge about trace elements and trace element species. Trace elements play essential roles in the maintenance of physiological processes. During the last decades there has been a great deal of scientific investigation about the function and binding of trace elements. The Journal of Trace Elements in Medicine and Biology focuses on the description and dissemination of scientific results concerning the role of trace elements with respect to their mode of action in health and disease and nutritional importance. Progress in the knowledge of the biological role of trace elements depends, however, on advances in trace elements chemistry. Thus the Journal of Trace Elements in Medicine and Biology will include only those papers that base their results on proven analytical methods.
Also, we only publish those articles in which the quality assurance regarding the execution of experiments and achievement of results is guaranteed.