Role of Rmd9p in 3′-end processing of mitochondrial 15S rRNA in Saccharomyces cerevisiae

IF 3.9 3区生物学 Q2 CELL BIOLOGY

Mitochondrion Pub Date : 2024-04-08 DOI:10.1016/j.mito.2024.101876

Jitendra Singh , Sudhir Singh , Elhassan Ali Fathi Emam , Umesh Varshney

引用次数: 0

Abstract

Ribosome biogenesis, involving processing/assembly of rRNAs and r-proteins is a vital process. In Saccharomyces cerevisiae mitochondria, ribosomal small subunit comprises 15S rRNA (15S). While the 15S 5′-end processing uses Ccm1p and Pet127p, the mechanisms of the 3′-end processing remain unclear. We reveal involvement of Rmd9p in safeguarding/processing 15S 3′-end. Rmd9p deficiency results in a cleavage at a position 183 nucleotides upstream of 15S 3′-end, and in the loss of the 3′-minor domain. Rmd9p binds to the sequences in the 3'-end region of 15S, and a genetic interaction between rmd9 and dss1 indicates that Rmd9p regulates/limits mtEXO activity during the 3′-end spacer processing.

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Rmd9p 在酿酒酵母线粒体 15S rRNA 3′端加工中的作用

核糖体的生物发生涉及 rRNA 和 r 蛋白的加工/组装，是一个至关重要的过程。在酿酒酵母线粒体中，核糖体小亚基包括 15S rRNA（15S）。15S 5′端处理过程使用了 Ccm1p 和 Pet127p，但 3′端处理的机制仍不清楚。我们发现Rmd9p参与了15S 3′-端的保护/处理。Rmd9p 缺乏会导致 15S 3′-末端上游 183 个核苷酸位置的裂解，并导致 3′-小结构域的缺失。Rmd9p与15S 3'端区域的序列结合，rmd9与dss1之间的遗传相互作用表明，Rmd9p在3′端间隔处理过程中调节/限制了mtEXO的活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Mitochondrion 生物-细胞生物学

CiteScore

9.40

自引率

4.50%

发文量

审稿时长

13.6 weeks

期刊介绍： Mitochondrion is a definitive, high profile, peer-reviewed international research journal. The scope of Mitochondrion is broad, reporting on basic science of mitochondria from all organisms and from basic research to pathology and clinical aspects of mitochondrial diseases. The journal welcomes original contributions from investigators working in diverse sub-disciplines such as evolution, biophysics, biochemistry, molecular and cell biology, genetics, pharmacology, toxicology, forensic science, programmed cell death, aging, cancer and clinical features of mitochondrial diseases.