Laser-irradiating infrared attenuated total reflection spectroscopy of articular cartilage: Potential and challenges for diagnosing osteoarthritis

P. Krebs , M. Nägele , P. Fomina , V. Virtanen , E. Nippolainen , R. Shaikh , I.O. Afara , J. Töyräs , I. Usenov , T. Sakharova , V. Artyushenko , V. Tafintseva , J.H. Solheim , B. Zimmermann , A. Kohler , O. König , S. Saarakkala , B. Mizaikoff
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引用次数: 0

Abstract

Objective

A prototype infrared attenuated total reflection (IR-ATR) laser spectroscopic system designed for in vivo classification of human cartilage tissue according to its histological health status during arthroscopic surgery is presented. Prior to real-world in vivo applications, this so-called osteoarthritis (OA) scanner has been tested at in vitro conditions revealing the challenges associated with complex sample matrices and the accordingly obtained sparse spectral datasets.

Methods

In vitro studies on human knee cartilage samples at different contact pressures (i.e., 0.2–0.5 ​MPa) allowed recording cartilage degeneration characteristic IR signatures comparable to in vivo conditions with high temporal resolution. Afterwards, the cartilage samples were assessed based on the clinically acknowledged osteoarthritis cartilage histopathology assessment (OARSI) system and correlated with the obtained sparse IR data.

Results

Amide and carbohydrate signal behavior was observed to be almost identical between the obtained sparse IR data and previously measured FTIR data used for sparse partial least squares discriminant analysis (SPLSDA) to identify the spectral regions relevant to cartilage condition. Contact pressures between 0.3 and 0.4 ​MPa seem to provide the best sparse IR spectra for cylindrical (d ​= ​3 ​mm) probe tips.

Conclusion

Laser-irradiating IR-ATR spectroscopy is a promising analytical technique for future arthroscopic applications to differentiate healthy and osteoarthritic cartilage tissue. However, this study also revealed that the flexible connection between the laser-based analyzer and the arthroscopic ATR-probe via IR-transparent fiberoptic cables may affect the robustness of the obtained IR data and requires further improvements.

关节软骨的激光照射红外衰减全反射光谱:诊断骨关节炎的潜力与挑战
目的介绍一种红外衰减全反射(IR-ATR)激光光谱系统原型,用于在关节镜手术中根据组织学健康状况对人体软骨组织进行体内分类。方法在不同接触压力(即 0.2-0.5 兆帕)下对人膝关节软骨样本进行体外研究,以高时间分辨率记录软骨退化特征红外特征,与体内条件相当。随后,根据临床公认的骨关节炎软骨组织病理学评估(OARSI)系统对软骨样本进行评估,并与获得的稀疏红外数据进行关联。结果 观察发现,获得的稀疏红外数据与之前用于稀疏偏最小二乘判别分析(SPLSDA)以识别与软骨状况相关的光谱区域的傅立叶变换红外数据之间,酰胺和碳水化合物信号行为几乎相同。对于圆柱形(d = 3 毫米)探针尖端,0.3 至 0.4 兆帕之间的接触压力似乎能提供最佳的稀疏红外光谱。然而,这项研究也揭示了激光分析仪与关节镜 ATR 探头之间通过红外透明光缆的柔性连接可能会影响所获红外数据的稳健性,因此需要进一步改进。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Osteoarthritis and cartilage open
Osteoarthritis and cartilage open Orthopedics, Sports Medicine and Rehabilitation
CiteScore
3.30
自引率
0.00%
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